| Literature DB >> 10382662 |
K C Jinneman1, J H Wetherington, W E Hill, C J Omiescinski, A M Adams, J M Johnson, B J Tenge, N L Dang, M M Wekell.
Abstract
An oligonucleotide-ligation assay (OLA) was developed and compared to a restriction fragment length polymorphism (RFLP) test for distinguishing between 294-bp polymerase chain reaction (PCR) amplification products of the 18S rRNA gene from Cyclospora and Eimeria spp. The PCR/OLA correctly distinguished between three Cyclospora, three E. tenella, and one E. mitis strains and the ratio of positive to negative spectrophotometric absorbance (A490) values for each strain ranged from 4.086 to 15.280 (median 9.5). PCR/OLA provides a rapid, reliable, spectrophotometric alternative to PCR/RFLP.Entities:
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Year: 1999 PMID： 10382662 DOI： 10.4315/0362-028x-62.6.682
Source DB: PubMed Journal: J Food Prot ISSN： 0362-028X Impact factor: 2.077