AIMS: To determine the relation between the stages of diabetic retinopathy (DR) and the levels of hepatocyte growth factor (HGF) and vascular endothelial growth factor (VEGF) in aqueous fluid and serum. METHODS: Levels of HGF and VEGF in serum and aqueous humour obtained during ocular surgery were measured by enzyme linked immunosorbent assay in 58 diabetic patients with 32 non-diabetic patients (NDM) as controls. The patients with diabetes were classified into three groups according to the stage of DR: no DR (NDR; 15 cases), non-proliferative DR (NPDR; six cases), and proliferative DR (PDR; 37 cases). RESULTS: No significant differences were found between any of the groups in serum concentrations of HGF or VEGF. The aqueous HGF levels increased with the stage of DR: NDM, median 397 pg/ml, range 133-930 pg/ml; NDR, 371 pg/ml, 142-1536 pg/ml; NPDR, 455 pg/ml, 162-1007 pg/ml; and PDR, 638 pg/ml, 187-2222 pg/ml. The aqueous VEGF levels in PDR (median 212 pg/ml, range 14-1216 pg/ml) were significantly higher than in NDM (105 pg/ml, 9-203 pg/ml), but aqueous HGF concentrations were unrelated to those of VEGF. CONCLUSION: The results of the present study suggest that both HGF and VEGF present in the ocular tissues may play important roles in the progression of DR.
AIMS: To determine the relation between the stages of diabetic retinopathy (DR) and the levels of hepatocyte growth factor (HGF) and vascular endothelial growth factor (VEGF) in aqueous fluid and serum. METHODS: Levels of HGF and VEGF in serum and aqueous humour obtained during ocular surgery were measured by enzyme linked immunosorbent assay in 58 diabeticpatients with 32 non-diabeticpatients (NDM) as controls. The patients with diabetes were classified into three groups according to the stage of DR: no DR (NDR; 15 cases), non-proliferative DR (NPDR; six cases), and proliferative DR (PDR; 37 cases). RESULTS: No significant differences were found between any of the groups in serum concentrations of HGF or VEGF. The aqueous HGF levels increased with the stage of DR: NDM, median 397 pg/ml, range 133-930 pg/ml; NDR, 371 pg/ml, 142-1536 pg/ml; NPDR, 455 pg/ml, 162-1007 pg/ml; and PDR, 638 pg/ml, 187-2222 pg/ml. The aqueous VEGF levels in PDR (median 212 pg/ml, range 14-1216 pg/ml) were significantly higher than in NDM (105 pg/ml, 9-203 pg/ml), but aqueous HGF concentrations were unrelated to those of VEGF. CONCLUSION: The results of the present study suggest that both HGF and VEGF present in the ocular tissues may play important roles in the progression of DR.
Authors: Y Nakamura; R Morishita; J Higaki; I Kida; M Aoki; A Moriguchi; K Yamada; S Hayashi; Y Yo; H Nakano; K Matsumoto; T Nakamura; T Ogihara Journal: J Hypertens Date: 1996-09 Impact factor: 4.844
Authors: R Morishita; S Nakamura; Y Nakamura; M Aoki; A Moriguchi; I Kida; Y Yo; K Matsumoto; T Nakamura; J Higaki; T Ogihara Journal: Diabetes Date: 1997-01 Impact factor: 9.461
Authors: M J Tolentino; J W Miller; E S Gragoudas; F A Jakobiec; E Flynn; K Chatzistefanou; N Ferrara; A P Adamis Journal: Ophthalmology Date: 1996-11 Impact factor: 12.079
Authors: Y Nakamura; R Morishita; S Nakamura; M Aoki; A Moriguchi; K Matsumoto; T Nakamura; J Higaki; T Ogihara Journal: Hypertension Date: 1996-09 Impact factor: 10.190
Authors: L P Aiello; R L Avery; P G Arrigg; B A Keyt; H D Jampel; S T Shah; L R Pasquale; H Thieme; M A Iwamoto; J E Park Journal: N Engl J Med Date: 1994-12-01 Impact factor: 91.245
Authors: Y Nakamura; R Morishita; J Higaki; I Kida; M Aoki; A Moriguchi; K Yamada; S Hayashi; Y Yo; K Matsumoto Journal: Biochem Biophys Res Commun Date: 1995-10-13 Impact factor: 3.575