Y Li1, N Wood, D Yellowlees, P K Donnelly. 1. Department of Surgery, North Queensland Clinical School, University of Queensland, Townsville, Australia.
Abstract
BACKGROUND: The urokinase receptor (uPAR) is important in the process of extracellular matrix degradation occurring during cancer cell invasion and metastasis. We wished to quantify uPAR on the surfaces of normal mammary epithelial cells (HMEC) and 6 well-known breast cancer cell lines using flow cytometry. MATERIALS AND METHODS: Cell surface uPAR was labelled with a monoclonal antibody, and this was detected with a fluorescent-labelled second antibody and accurately measured using flow cytometry. The measured fluorescent signals of the stained cells were interpolated with those of Quantum Simply Cellular bead standards to determine the number of uPAR sites per cell. RESULTS: The breast cancer cell lines ranged from 13,700 to 50,800 uPAR sites per cell, whilst HMEC cells had only 2,500 sites. CONCLUSIONS: This simple and reliable method showed that the expression of cell surface uPAR is higher in the breast cancer cell lines than in the normal mammary cells.
BACKGROUND: The urokinase receptor (uPAR) is important in the process of extracellular matrix degradation occurring during cancer cell invasion and metastasis. We wished to quantify uPAR on the surfaces of normal mammary epithelial cells (HMEC) and 6 well-known breast cancer cell lines using flow cytometry. MATERIALS AND METHODS: Cell surface uPAR was labelled with a monoclonal antibody, and this was detected with a fluorescent-labelled second antibody and accurately measured using flow cytometry. The measured fluorescent signals of the stained cells were interpolated with those of Quantum Simply Cellular bead standards to determine the number of uPAR sites per cell. RESULTS: The breast cancer cell lines ranged from 13,700 to 50,800 uPAR sites per cell, whilst HMEC cells had only 2,500 sites. CONCLUSIONS: This simple and reliable method showed that the expression of cell surface uPAR is higher in the breast cancer cell lines than in the normal mammary cells.
Authors: Lily Yang; Zehong Cao; Hari Krishna Sajja; Hui Mao; Liya Wang; Huaying Geng; Hengyi Xu; Tieshan Jiang; William C Wood; Shuming Nie; Y Andrew Wang Journal: J Biomed Nanotechnol Date: 2008-12-01 Impact factor: 4.099
Authors: Lily Yang; Hari Krishna Sajja; Zehong Cao; Weiping Qian; Laura Bender; Adam I Marcus; Malgorzata Lipowska; William C Wood; Y Andrew Wang Journal: Theranostics Date: 2013-12-17 Impact factor: 11.556