H Li1, Q Mao, Q Li. 1. Center of Infectious Diseases, Southwest Hospital, Third Military Medical University, Chongqing.
Abstract
OBJECTIVE: To study inhibitory effect of an antisense oligodeoxynucleotide (ASODN) and its phosphorothioate (S-ASODN) on replication and expression of HDV in H1 delta 9 cell. METHODS: In previous studies, it was proved that the ASODNs which are complementary to genomic HDV ribozyme self-cleavage site and stem I regions can inhibit availably genomic HDV ribozyme activity. In the present study, a 15-mer ASODN and S-ASODS which are complementary to this region (nucleotide 684-698) were added to medium of cultured H1 delta 9 cell. HDAg and HDV cDNA were detected by ELISA and Dot blot hybridization. RESULTS: Twenty-fourth hour after 6 mumol/L ASODN and S-ASODN were added, both the secreting amount of HDAg in the supernatant and HDV RNA were inhibited. The inhibiting rates were 76.14% and 84.50% respectively. When the concentration of S-ASODN was 2, 4, 6 mumol/L, the inhibiting rate showed the feature of dosage dependence. Inhibitory effect of ASODN and S-ASODN were similar in the same dosage. CONCLUSION: The results suggest that ASODN and S-ASODN can availably inhibit replication and expression of HDV in H1 delta 9 cell.
OBJECTIVE: To study inhibitory effect of an antisense oligodeoxynucleotide (ASODN) and its phosphorothioate (S-ASODN) on replication and expression of HDV in H1 delta 9 cell. METHODS: In previous studies, it was proved that the ASODNs which are complementary to genomic HDV ribozyme self-cleavage site and stem I regions can inhibit availably genomic HDV ribozyme activity. In the present study, a 15-mer ASODN and S-ASODS which are complementary to this region (nucleotide 684-698) were added to medium of cultured H1 delta 9 cell. HDAg and HDV cDNA were detected by ELISA and Dot blot hybridization. RESULTS: Twenty-fourth hour after 6 mumol/L ASODN and S-ASODN were added, both the secreting amount of HDAg in the supernatant and HDV RNA were inhibited. The inhibiting rates were 76.14% and 84.50% respectively. When the concentration of S-ASODN was 2, 4, 6 mumol/L, the inhibiting rate showed the feature of dosage dependence. Inhibitory effect of ASODN and S-ASODN were similar in the same dosage. CONCLUSION: The results suggest that ASODN and S-ASODN can availably inhibit replication and expression of HDV in H1 delta 9 cell.