OBJECTIVE: To find out whether tumour DNA content correlates with allelic loss of p53 and other pathological features in primary colorectal carcinomas. DESIGN: Ongoing prospective study. SETTING: University hospital, Italy. SUBJECTS: 128 patients who had undergone radical resections for colorectal carcinoma. INTERVENTIONS: Flow cytometric measurement of tumour DNA content and detection of allelic loss on the short arm of chromosome 17 by Southern blot (restriction fragment length polymorphism) analysis in fresh tumour specimens. MAIN OUTCOME MEASURES: Correlation between DNA ploidy and deletion of p53, as well as between these two genetic events and clinicopathological variables. RESULTS: Interpretable DNA histograms were obtained for 122 tumour specimens. Forty-three tumours (35%) were diploid and 79 (65%) aneuploid. The diploid tumours were significantly more common in the proximal colon (from the caecum to the splenic flexure) than in the distal colon (from the descending colon to the rectum) (p = 0.002). The allelic state on the short arm of chromosome 17 was evaluated in 80 heterozygous patients. Forty-four tumour specimens (55%) showed deletion of 17p. Allelic loss of p53 was significantly more common in the distal and rectal tumours than in the proximal ones (p < 0.0001). Aneuploidy was more common among those tumours which had shown deletion of p53 than in those that had not (p = 0.0008). CONCLUSIONS: DNA aneuploidy was significantly associated with the deletion of the p53 gene. This suggests that the functional loss of p53 may favour the growth and establishment of an aneuploid cell population within tumours. Tumours of the proximal and distal colon differ in their genetic nature.
OBJECTIVE: To find out whether tumour DNA content correlates with allelic loss of p53 and other pathological features in primary colorectal carcinomas. DESIGN: Ongoing prospective study. SETTING: University hospital, Italy. SUBJECTS: 128 patients who had undergone radical resections for colorectal carcinoma. INTERVENTIONS: Flow cytometric measurement of tumour DNA content and detection of allelic loss on the short arm of chromosome 17 by Southern blot (restriction fragment length polymorphism) analysis in fresh tumour specimens. MAIN OUTCOME MEASURES: Correlation between DNA ploidy and deletion of p53, as well as between these two genetic events and clinicopathological variables. RESULTS: Interpretable DNA histograms were obtained for 122 tumour specimens. Forty-three tumours (35%) were diploid and 79 (65%) aneuploid. The diploid tumours were significantly more common in the proximal colon (from the caecum to the splenic flexure) than in the distal colon (from the descending colon to the rectum) (p = 0.002). The allelic state on the short arm of chromosome 17 was evaluated in 80 heterozygous patients. Forty-four tumour specimens (55%) showed deletion of 17p. Allelic loss of p53 was significantly more common in the distal and rectal tumours than in the proximal ones (p < 0.0001). Aneuploidy was more common among those tumours which had shown deletion of p53 than in those that had not (p = 0.0008). CONCLUSIONS: DNA aneuploidy was significantly associated with the deletion of the p53 gene. This suggests that the functional loss of p53 may favour the growth and establishment of an aneuploid cell population within tumours. Tumours of the proximal and distal colon differ in their genetic nature.
Authors: Mijung Kwon; Genesaret Rubio; Nicholas Nolan; Peter Auteri; Jean Arly Volmar; Asha Adem; Parisa Javidian; Zhongren Zhou; Michael P Verzi; Sharon R Pine; Steven K Libutti Journal: Cancer Res Date: 2021-08-20 Impact factor: 12.701
Authors: Akshay Narkar; Blake A Johnson; Pandurang Bharne; Jin Zhu; Veena Padmanaban; Debojyoti Biswas; Andrew Fraser; Pablo A Iglesias; Andrew J Ewald; Rong Li Journal: Cell Rep Date: 2021-03-23 Impact factor: 9.423
Authors: Carlos López-García; Laurent Sansregret; Enric Domingo; Nicholas McGranahan; Sebastijan Hobor; Nicolai Juul Birkbak; Stuart Horswell; Eva Grönroos; Francesco Favero; Andrew J Rowan; Nicholas Matthews; Sharmin Begum; Benjamin Phillimore; Rebecca Burrell; Dahmane Oukrif; Bradley Spencer-Dene; Michal Kovac; Gordon Stamp; Aengus Stewart; Havard Danielsen; Marco Novelli; Ian Tomlinson; Charles Swanton Journal: Cancer Cell Date: 2017-01-09 Impact factor: 38.585
Authors: A P McGlynn; G R Wasson; S O'Reilly; V J McKelvey-Martin; H McNulty; J J Strain; G McKerr; F Mullan; N Mahmud; J Scott; D G Weir; C S Downes Journal: Br J Cancer Date: 2003-03-24 Impact factor: 7.640