OBJECTIVE: To identify rheumatoid arthritis (RA) specific autoantibody and its antigen in the human osteoblast-like cell line, MG-63. METHODS: MG-63 cell extract was subjected to western blotting by using RA and normal serum samples as probes. The autoantigen was purified and its N-terminal sequence was determined by automated Edman degradation. The reactivity of denatured aldolase A was evaluated by immunoblotting. Screening by enzyme linked immunosorbent assay (ELISA) using the autoantibody was performed. RESULTS: 40 kDa protein was found only in the RA serum samples and it was identified as aldolase A. A polyclonal antibody for rabbit muscle aldolase A bound to the 40 kDa protein and reacted in preference with the denatured enzyme. Using ELISA for denatured rabbit aldolase A, the autoantibody was found in approximately 10% of RA patients, whereas it was not found in the other arthropathy and healthy adults. CONCLUSION: This 40 kDa anti-aldolase A autoantibody, which was identified only in serum samples of RA patients with severe bone erosion, could be related to a certain event that induces RA specific joint destructions.
OBJECTIVE: To identify rheumatoid arthritis (RA) specific autoantibody and its antigen in the human osteoblast-like cell line, MG-63. METHODS: MG-63 cell extract was subjected to western blotting by using RA and normal serum samples as probes. The autoantigen was purified and its N-terminal sequence was determined by automated Edman degradation. The reactivity of denatured aldolase A was evaluated by immunoblotting. Screening by enzyme linked immunosorbent assay (ELISA) using the autoantibody was performed. RESULTS: 40 kDa protein was found only in the RA serum samples and it was identified as aldolase A. A polyclonal antibody for rabbit muscle aldolase A bound to the 40 kDa protein and reacted in preference with the denatured enzyme. Using ELISA for denatured rabbit aldolase A, the autoantibody was found in approximately 10% of RApatients, whereas it was not found in the other arthropathy and healthy adults. CONCLUSION: This 40 kDa anti-aldolase A autoantibody, which was identified only in serum samples of RApatients with severe bone erosion, could be related to a certain event that induces RA specific joint destructions.
Authors: F C Arnett; S M Edworthy; D A Bloch; D J McShane; J F Fries; N S Cooper; L A Healey; S R Kaplan; M H Liang; H S Luthra Journal: Arthritis Rheum Date: 1988-03
Authors: K Terato; Y Shimozuru; K Katayama; Y Takemitsu; I Yamashita; M Miyatsu; K Fujii; M Sagara; S Kobayashi; M Goto Journal: Arthritis Rheum Date: 1990-10
Authors: G Steiner; K Hartmuth; K Skriner; I Maurer-Fogy; A Sinski; E Thalmann; W Hassfeld; A Barta; J S Smolen Journal: J Clin Invest Date: 1992-09 Impact factor: 14.808
Authors: James S McCarthy; Moira Wieseman; Joe Tropea; David Kaslow; David Abraham; Sara Lustigman; Rocky Tuan; Ronald H Guderian; Thomas B Nutman Journal: Infect Immun Date: 2002-02 Impact factor: 3.441