Antibacterial and antifungal activity of small protein of Indigofera oblongifolia leaves.

Four fractions from the leaves of Indigofera oblongifolia were obtained on Sephadex G-25 column chromatography. A single bands of these fractions were detected on Poly-acrylamide SDS gel electrophoresis. An antibacterial action of small protein peptide was tested against Escherichia coli, Klebsiella aerogenes, Kl. pneumoniae. Staphyllococcus aureus, and Bacillus subtilis. Peptide 3 showed strong inhibitory activity against B. subtilis and Aspergillus niger but clear zone of inhibition was also noted against A. fumigatus. Peptide 4 showed significant inhibitory zone against Kl. pneumoniae, S. aureus, B. subtilis. A. fumigatus, A. niger and A. flavus.