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Literature DB >> 10361087

A chemogenetic approach to RNA function/structure analysis.

Abstract

Almost two dozen nucleotide analogs have been synthesized with alpha-phosphorothioate-tagged triphosphates and utilized in an interference modification approach termed Nucleotide Analog Interference Mapping. This method has made it possible to determine the chemical basis of RNA function and structure, including the identification of new rules for RNA helix packing, the functional analysis of a binding site for monovalent metal ions within RNA and the characterization of the catalytic mechanism of RNA enzymes.

Entities: Chemical

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Year: 1999 PMID： 10361087 DOI： 10.1016/S0959-440X(99)80046-3

Source DB: PubMed Journal: Curr Opin Struct Biol ISSN： 0959-440X Impact factor: 6.809

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Cited

20 in total

Review 1. Recent advances in the elucidation of the mechanisms of action of ribozymes.

Authors: Y Takagi; M Warashina; W J Stec; K Yoshinari; K Taira
Journal: Nucleic Acids Res Date: 2001-05-01 Impact factor: 16.971

2. Selecting rRNA binding sites for the ribosomal proteins L4 and L6 from randomly fragmented rRNA: application of a method called SERF.

Authors: U Stelzl; C M Spahn; K H Nierhaus
Journal: Proc Natl Acad Sci U S A Date: 2000-04-25 Impact factor: 11.205

3. A short fragment of 23S rRNA containing the binding sites for two ribosomal proteins, L24 and L4, is a key element for rRNA folding during early assembly.

Authors: U Stelzl; K H Nierhaus
Journal: RNA Date: 2001-04 Impact factor: 4.942

A chemogenetic approach to RNA function/structure analysis.

Review 1. Recent advances in the elucidation of the mechanisms of action of ribozymes.

2. Selecting rRNA binding sites for the ribosomal proteins L4 and L6 from randomly fragmented rRNA: application of a method called SERF.

3. A short fragment of 23S rRNA containing the binding sites for two ribosomal proteins, L24 and L4, is a key element for rRNA folding during early assembly.

4. Quick two-step RNA ligation employing periodate oxidation.

5. RNA-structural mimicry in Escherichia coli ribosomal protein L4-dependent regulation of the S10 operon.

6. The functional anatomy of an intrinsic transcription terminator.

7. Quantitation of free energy profiles in RNA-ligand interactions by nucleotide analog interference mapping.

8. Linking the group II intron catalytic domains: tertiary contacts and structural features of domain 3.

Review 9. RNA nanotechnology: engineering, assembly and applications in detection, gene delivery and therapy.

10. Dissecting RNA folding by nucleotide analog interference mapping (NAIM).