| Literature DB >> 10360419 |
O O Omole1, D R Brocks, G Nappert, J M Naylor, G A Zello.
Abstract
Two high-performance liquid chromatographic methods are described for the determination of lactic acid and its enantiomers in calf serum. A 300x8.0 mm I.D. column packed with sulfonated styrene-divinylbenzene copolymer and a 50x4.6 mm ODS column with N,N-dioctyl-L-alanine were used. UV detection was at 205 and 236 nm for the non-chiral and chiral assays, respectively. Both assays demonstrated excellent linear relationships between peak area ratios and serum concentrations over a range of 0.5 to 20 mM, based on 100 microl bovine serum. Recovery was complete. Inter- and within-batch bias and relative standard deviation were <15%.Entities:
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Year: 1999 PMID： 10360419 DOI： 10.1016/s0378-4347(99)00072-9
Source DB: PubMed Journal: J Chromatogr B Biomed Sci Appl ISSN： 1387-2273