Literature DB >> 10359847

AtPCS1, a phytochelatin synthase from Arabidopsis: isolation and in vitro reconstitution.

O K Vatamaniuk1, S Mari, Y P Lu, P A Rea.   

Abstract

Phytochelatins, a class of posttranslationally synthesized peptides, play a pivotal role in heavy metal, primarily Cd2+, tolerance in plants and fungi by chelating these substances and decreasing their free concentrations. Derived from glutathione and related thiols by the action of gamma-glutamylcysteine dipeptidyl transpeptidases (phytochelatin synthases; EC 2.3.2.15), phytochelatins consist of repeating units of gamma-glutamylcysteine followed by a C-terminal Gly, Ser, or beta-Ala residue [poly-(gamma-Glu-Cys)n-Xaa]. Here we report the suppression cloning of a cDNA (AtPCS1) from Arabidopsis thaliana encoding a 55-kDa soluble protein that enhances heavy-metal tolerance and elicits Cd2+-activated phytochelatin accumulation when expressed in Saccharomyces cerevisiae. On the basis of these properties and the sufficiency of immunoaffinity-purified epitope-tagged AtPCS1 polypeptide for high rates of Cd2+-activated phytochelatin synthesis from glutathione in vitro, AtPCS1 is concluded to encode the enzyme phytochelatin synthase.

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Year:  1999        PMID： 10359847      PMCID： PMC22073          DOI： 10.1073/pnas.96.12.7110

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  29 in total

1.  AtMRP1 gene of Arabidopsis encodes a glutathione S-conjugate pump: isolation and functional definition of a plant ATP-binding cassette transporter gene.

Authors:  Y P Lu; Z S Li; P A Rea
Journal:  Proc Natl Acad Sci U S A       Date:  1997-07-22       Impact factor: 11.205

2.  Applications of high efficiency lithium acetate transformation of intact yeast cells using single-stranded nucleic acids as carrier.

Authors:  R D Gietz; R H Schiestl
Journal:  Yeast       Date:  1991-04       Impact factor: 3.239

3.  Tolerance to toxic metals by a gene family of phytochelatin synthases from plants and yeast.

Authors:  S Clemens; E J Kim; D Neumann; J I Schroeder
Journal:  EMBO J       Date:  1999-06-15       Impact factor: 11.598

4.  Phytochelatins, the heavy-metal-binding peptides of plants, are synthesized from glutathione by a specific gamma-glutamylcysteine dipeptidyl transpeptidase (phytochelatin synthase).

Authors:  E Grill; S Löffler; E L Winnacker; M H Zenk
Journal:  Proc Natl Acad Sci U S A       Date:  1989-09       Impact factor: 11.205

5.  Subcellular localization of cadmium and cadmium-binding peptides in tobacco leaves : implication of a transport function for cadmium-binding peptides.

Authors:  R Vögeli-Lange; G J Wagner
Journal:  Plant Physiol       Date:  1990-04       Impact factor: 8.340

6.  A new pathway for vacuolar cadmium sequestration in Saccharomyces cerevisiae: YCF1-catalyzed transport of bis(glutathionato)cadmium.

Authors:  Z S Li; Y P Lu; R G Zhen; M Szczypka; D J Thiele; P A Rea
Journal:  Proc Natl Acad Sci U S A       Date:  1997-01-07       Impact factor: 11.205

7.  The role of glutathione in copper metabolism and toxicity.

Authors:  J H Freedman; M R Ciriolo; J Peisach
Journal:  J Biol Chem       Date:  1989-04-05       Impact factor: 5.157

8.  Transcriptional activation mediated by the yeast AP-1 protein is required for normal cadmium tolerance.

Authors:  J A Wemmie; A L Wu; K D Harshman; C S Parker; W S Moye-Rowley
Journal:  J Biol Chem       Date:  1994-05-20       Impact factor: 5.157

9.  The yeast cadmium factor protein (YCF1) is a vacuolar glutathione S-conjugate pump.

Authors:  Z S Li; M Szczypka; Y P Lu; D J Thiele; P A Rea
Journal:  J Biol Chem       Date:  1996-03-15       Impact factor: 5.157

10.  Heavy metal tolerance in the fission yeast requires an ATP-binding cassette-type vacuolar membrane transporter.

Authors:  D F Ortiz; L Kreppel; D M Speiser; G Scheel; G McDonald; D W Ow
Journal:  EMBO J       Date:  1992-10       Impact factor: 11.598
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  71 in total

1.  Phytochelatins are involved in differential arsenate tolerance in Holcus lanatus.

Authors:  J Hartley-Whitaker; G Ainsworth; R Vooijs; W Ten Bookum; H Schat; A A Meharg
Journal:  Plant Physiol       Date:  2001-05       Impact factor: 8.340

2.  Gene expression in cadmium-tolerant Datura innoxia: detection and characterization of cDNAs induced in response to Cd2+.

Authors:  Maggie Louie; Nathan Kondor; Jane G DeWitt
Journal:  Plant Mol Biol       Date:  2003-05       Impact factor: 4.076

3.  Long-distance root-to-shoot transport of phytochelatins and cadmium in Arabidopsis.

Authors:  Ji-Ming Gong; David A Lee; Julian I Schroeder
Journal:  Proc Natl Acad Sci U S A       Date:  2003-08-08       Impact factor: 11.205

Review 4.  Weeds, worms, and more. Papain's long-lost cousin, phytochelatin synthase.

Authors:  Philip A Rea; Olena K Vatamaniuk; Daniel J Rigden
Journal:  Plant Physiol       Date:  2004-09       Impact factor: 8.340

5.  Arabidopsis and the genetic potential for the phytoremediation of toxic elemental and organic pollutants.

Authors:  Christopher S Cobbett; Richard B Meagher
Journal:  Arabidopsis Book       Date:  2002-04-04

6.  Cadmium tolerance and accumulation in Indian mustard is enhanced by overexpressing gamma-glutamylcysteine synthetase.

Authors:  Y L Zhu; E A Pilon-Smits; A S Tarun; S U Weber; L Jouanin; N Terry
Journal:  Plant Physiol       Date:  1999-12       Impact factor: 8.340

7.  Feedback inhibition by thiols outranks glutathione depletion: a luciferase-based screen reveals glutathione-deficient γ-ECS and glutathione synthetase mutants impaired in cadmium-induced sulfate assimilation.

Authors:  Timothy O Jobe; Dong-Yul Sung; Garo Akmakjian; Allis Pham; Elizabeth A Komives; David G Mendoza-Cózatl; Julian I Schroeder
Journal:  Plant J       Date:  2012-03-31       Impact factor: 6.417

8.  The shoot-specific expression of gamma-glutamylcysteine synthetase directs the long-distance transport of thiol-peptides to roots conferring tolerance to mercury and arsenic.

Authors:  Yujing Li; Om Parkash Dankher; Laura Carreira; Aaron P Smith; Richard B Meagher
Journal:  Plant Physiol       Date:  2006-03-31       Impact factor: 8.340

9.  Phytochelatin synthesis is essential for the detoxification of excess zinc and contributes significantly to the accumulation of zinc.

Authors:  Pierre Tennstedt; Daniel Peisker; Christoph Böttcher; Aleksandra Trampczynska; Stephan Clemens
Journal:  Plant Physiol       Date:  2008-12-12       Impact factor: 8.340

10.  Centaurin-like protein Cnt5 contributes to arsenic and cadmium resistance in fission yeast.

Authors:  Ajay Amar Vashisht; Patrick Joseph Kennedy; Paul Russell
Journal:  FEMS Yeast Res       Date:  2008-12-06       Impact factor: 2.796
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