Laminar distribution of pheromone-receptive neurons in rat vomeronasal epithelium.

1. Responses of vomeronasal sensory neurons to urine excreted from rats, mice and hamsters were studied by the on-cell patch clamp method in slices of sensory epithelium from female Wistar rats. 2. The urine excreted from male and female Wistar rats, male Donryu rats and male C57BL/6 mice induced relatively large responses, while urine from male Sprague-Dawley rats and male Syrian hamsters induced small responses. 3. Of the 62 neurons responding to urine, 57 responded to only one of the urine preparations. 4. The sensory neurons that responded to the male Wistar urine were localized in the apical position of the epithelium where one type of GTP-binding protein, Gi2alpha, is selectively expressed. The neurons in the basal position of the epithelium, which express Goalpha, responded to urine from the other animals. 5. This study demonstrates that sensory neurons responsive to different urinary pheromones are localized in a segregated layer in the rat vomeronasal sensory epithelium.