Literature DB >> 10354855

Detection of ileS-2 gene encoding mupirocin resistance in methicillin-resistant Staphylococcus aureus by multiplex PCR.

E L Nunes1, K R dos Santos, P J Mondino, M do C Bastos, M Giambiagi-deMarval.   

Abstract

The presence of the ileS-2 gene, responsible for mupirocin resistance, in clinical isolates of methicillin-resistant Staphylococcus aureus was determined by multiplex polymerase chain reaction. Three pairs of primers were used, which yielded specific fragments of femA (encoding a unique feature of S. aureus), mecA (encoding resistance to methicillin) and ileS-2 genes. The multiplex polymerase chain reaction system is an easy and time-saving technique that, together with a rapid method for DNA extraction by boiling, may be incorporated as a routine analysis in clinical diagnostic laboratories.

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Year:  1999        PMID： 10354855     DOI： 10.1016/s0732-8893(99)00021-8

Source DB:  PubMed          Journal:  Diagn Microbiol Infect Dis        ISSN： 0732-8893            Impact factor:   2.803


  17 in total

1.  Characterization of the mupA gene in strains of methicillin-resistant Staphylococcus aureus with a low level of resistance to mupirocin.

Authors:  S Fujimura; A Watanabe; D Beighton
Journal:  Antimicrob Agents Chemother       Date:  2001-02       Impact factor: 5.191

2.  Multiplex PCR strategy for rapid identification of structural types and variants of the mec element in methicillin-resistant Staphylococcus aureus.

Authors:  Duarte C Oliveira; Hermínia de Lencastre
Journal:  Antimicrob Agents Chemother       Date:  2002-07       Impact factor: 5.191

3.  Coagulase-negative staphylococci: comparison of phenotypic and genotypic oxacillin susceptibility tests and evaluation of the agar screening test by using different concentrations of oxacillin.

Authors:  Rosana B R Ferreira; Natalia L P Iorio; Karoline L Malvar; Ana Paula F Nunes; Leila S Fonseca; Carla C R Bastos; Kátia R N Santos
Journal:  J Clin Microbiol       Date:  2003-08       Impact factor: 5.948

4.  Isoleucyl-tRNA synthetase mutations in Staphylococcus aureus clinical isolates and in vitro selection of low-level mupirocin-resistant strains.

Authors:  Shigeru Fujimura; Yutaka Tokue; Akira Watanabe
Journal:  Antimicrob Agents Chemother       Date:  2003-10       Impact factor: 5.191

5.  A decline in mupirocin resistance in methicillin-resistant Staphylococcus aureus accompanied administrative control of prescriptions.

Authors:  Elaine S Walker; Foster Levy; Mahmoud Shorman; Gerard David; Jehad Abdalla; Felix A Sarubbi
Journal:  J Clin Microbiol       Date:  2004-06       Impact factor: 5.948

Review 6.  Current and Emerging Topical Antibacterials and Antiseptics: Agents, Action, and Resistance Patterns.

Authors:  Deborah A Williamson; Glen P Carter; Benjamin P Howden
Journal:  Clin Microbiol Rev       Date:  2017-07       Impact factor: 26.132

7.  Multiplex PCR for simultaneous identification of Staphylococcus aureus and detection of methicillin and mupirocin resistance.

Authors:  E Pérez-Roth; F Claverie-Martín; J Villar; S Méndez-Alvarez
Journal:  J Clin Microbiol       Date:  2001-11       Impact factor: 5.948

8.  New quadriplex PCR assay for detection of methicillin and mupirocin resistance and simultaneous discrimination of Staphylococcus aureus from coagulase-negative staphylococci.

Authors:  Kunyan Zhang; Jennifer Sparling; Barbara L Chow; Sameer Elsayed; Zafar Hussain; Deirdre L Church; Daniel B Gregson; Thomas Louie; John M Conly
Journal:  J Clin Microbiol       Date:  2004-11       Impact factor: 5.948

Review 9.  Molecular diagnostics of clinically important staphylococci.

Authors:  J Stepán; R Pantůcek; J Doskar
Journal:  Folia Microbiol (Praha)       Date:  2004       Impact factor: 2.099

10.  Prevalence of mupirocin resistance in Staphylococcus pseudintermedius.

Authors:  Stacey M Godbeer; Randi M Gold; Sara D Lawhon
Journal:  J Clin Microbiol       Date:  2014-01-29       Impact factor: 5.948
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