Literature DB >> 10350572

Influence of serum-free culture conditions on steroid 5alpha-reductase mRNA expression in rat C6 glioma cells.

K Morita1, Y Tsuruo, K Ishimura, S Her, R A Bell, D L Wong.   

Abstract

Immunocytochemical studies previously showed that serum deprivation resulted in the appearance of steroid 5alpha-reductase (5alpha-R) in the cytoplasm of rat C6 glioma cells. To determine whether this increase in cytoplasmic 5alpha-R was due to changes in 5alpha-R gene expression, the effect of serum deprivation on 5alpha-R mRNA expression was examined. No significant change in the mRNA levels was observed in cells grown in serum-free culture medium. Therefore, the appearance of 5alpha-R immunoreactivity in the cell cytoplasm observed under serum-free conditions is probably not due to changes in 5alpha-R gene expression. Copyright 1999 Elsevier Science B.V.

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Year:  1999        PMID： 10350572     DOI： 10.1016/s0006-8993(99)01292-5

Source DB:  PubMed          Journal:  Brain Res        ISSN： 0006-8993            Impact factor:   3.252


  7 in total

1.  High salt culture conditions suppress proliferation of rat C6 glioma cell by arresting cell-cycle progression at S-phase.

Authors:  Hideki Arimochi; Kyoji Morita
Journal:  J Mol Neurosci       Date:  2005       Impact factor: 3.444

2.  Trichostatin A enhances glutamate transporter GLT-1 mRNA levels in C6 glioma cells via neurosteroid-mediated cell differentiation.

Authors:  Mari Itoh; Takara Hiroi; Naoyoshi Nishibori; Takefumi Sagara; Song Her; Mi-Sook Lee; Kyoji Morita
Journal:  J Mol Neurosci       Date:  2012-06-19       Impact factor: 3.444

3.  Progesterone pretreatment enhances serotonin-stimulated BDNF gene expression in rat c6 glioma cells through production of 5alpha-reduced neurosteroids.

Authors:  Kyoji Morita; Song Her
Journal:  J Mol Neurosci       Date:  2008-01-25       Impact factor: 3.444

4.  High salt culture conditions inhibit serum- and NGF- but not PMA-induced Egr-1 gene transcription in rat C6 glioma cells.

Authors:  Kyoji Morita
Journal:  J Mol Neurosci       Date:  2007       Impact factor: 3.444

5.  Adrenergic activation of steroid 5alpha-reductase gene expression in rat C6 glioma cells: involvement of cyclic amp/protein kinase A-mediated signaling pathway.

Authors:  Kyoji Morita; Hideki Arimochi; Yoshihiro Tsuruo
Journal:  J Mol Neurosci       Date:  2004       Impact factor: 3.444

6.  Possible relation of hemin-induced HO-1 expression to the upregulation of VEGF and BDNF mRNA levels in rat C6 glioma cells.

Authors:  Kyoji Morita; Mi-Sook Lee; Song Her
Journal:  J Mol Neurosci       Date:  2008-10-21       Impact factor: 3.444

7.  Short-term sleep disturbance enhances brain-derived neurotrophic factor gene expression in rat hippocampus by acting as internal stressor.

Authors:  Hiroaki Fujihara; Hiroyoshi Sei; Yusuke Morita; Yoichi Ueta; Kyoji Morita
Journal:  J Mol Neurosci       Date:  2003       Impact factor: 3.444
  7 in total

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