Literature DB >> 10347767

A polymerase chain reaction-based assay to identify genotype F of hepatitis B virus.

M T Moraes1, C Niel, S A Gomes.   

Abstract

We have developed a polymerase chain reaction (PCR) assay which distinguishes genotype F from the other genotypes of hepatitis B virus (HBV). The method was used to characterize HBV strains isolated in urban areas of the Brazilian Amazon. DNA was amplified in 54 of a total of 78 HBsAg-positive serum samples, using universal, non-genotype-specific primers. Only 4 (7.4%) were identified as genotype F by our genotype-specific PCR assay. This proportion is notably lower than that previously reported in Argentina, Venezuela, Peru, and Central America.

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Year:  1999        PMID： 10347767     DOI： 10.1590/s0100-879x1999000100006

Source DB:  PubMed          Journal:  Braz J Med Biol Res        ISSN： 0100-879X            Impact factor:   2.590


  7 in total

1.  Detection of mixed populations of wild-type and YMDD hepatitis B variants by pyrosequencing in acutely and chronically infected patients.

Authors:  Francisco C A Mello; Bárbara V Lago; Lia L Lewis-Ximenez; Carlos A Fernandes; Selma A Gomes
Journal:  BMC Microbiol       Date:  2012-06-06       Impact factor: 3.605

2.  Low frequency of mutations in the core promoter and precore regions of hepatitis B virus in anti-HBe positive Brazilian carriers.

Authors:  L De Castro; C Niel; S A Gomes
Journal:  BMC Microbiol       Date:  2001-07-06       Impact factor: 3.605

3.  Phylogeography and evolutionary history of hepatitis B virus genotype F in Brazil.

Authors:  Francisco C A Mello; Oscar C Araujo; Barbara V Lago; Ana Rita C Motta-Castro; Marcia Terezinha B Moraes; Selma A Gomes; Gonzalo Bello; Natalia M Araujo
Journal:  Virol J       Date:  2013-07-16       Impact factor: 4.099

4.  Molecular epidemiology of hepatitis B and hepatitis delta viruses circulating in the Western Amazon region, North Brazil.

Authors:  Myuki Alfaia Esashika Crispim; Nelson Abrahim Fraiji; Sonia Cordeiro Campello; Nicolaus Albert Schriefer; Mariane Martins Araújo Stefani; Dagmar Kiesslich
Journal:  BMC Infect Dis       Date:  2014-02-21       Impact factor: 3.090

5.  Hepatitis B virus genotypes circulating in Brazil: molecular characterization of genotype F isolates.

Authors:  Francisco C A Mello; Francisco J D Souto; Leticia C Nabuco; Cristiane A Villela-Nogueira; Henrique Sergio M Coelho; Helena Cristina F Franz; Joao Carlos P Saraiva; Helaine A Virgolino; Ana Rita C Motta-Castro; Mabel M M Melo; Regina M B Martins; Selma A Gomes
Journal:  BMC Microbiol       Date:  2007-11-23       Impact factor: 3.605

6.  Monitoring the emergence of HBV resistance mutations by HBV-RNA pyrosequencing.

Authors:  Marcelle Bottecchia; Halime Silva Barcaiu; Lia Laura Lewis-Ximenez; Sergio da Silva E Mouta Junior; Marcia Terezinha Baroni de Moraes
Journal:  Braz J Infect Dis       Date:  2015-12-31       Impact factor: 3.257

7.  Screening for hepatitis B virus in Maracanã workers.

Authors:  Marcelle Bottecchia; Juliana Custódio Miguel; Elisangela Ferreira da Silva; Cleber Ferreira Ginuíno; Marcia Terezinha Baroni de Moraes e Souza
Journal:  Braz J Infect Dis       Date:  2014-10-13       Impact factor: 3.257
  7 in total

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