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Literature DB >> 10347071

High-level production of heterologous protein by engineered yeasts grown in cottage cheese whey.

C Maullu¹, G Lampis, A Desogus, A Ingianni, G M Rossolini, R Pompei.

Abstract

Cottage cheese whey is a cheese industry by-product still rich in proteins and lactose. Its recycling is seldom cost-effective. In this work we show that the lactose-utilizing yeast Kluyveromyces lactis, engineered for production of recombinant human lysozyme, can be grown in cottage cheese whey, resulting in high-level production of the heterologous protein (125 microg/ml).

Entities: Chemical Gene Species

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Year: 1999 PMID： 10347071 PMCID： PMC91406 DOI： 10.1128/AEM.65.6.2745-2747.1999

Source DB: PubMed Journal: Appl Environ Microbiol ISSN： 0099-2240 Impact factor: 4.792

16 in total

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High-level production of heterologous protein by engineered yeasts grown in cottage cheese whey.

1. Production of bakers' yeast in cheese whey ultrafiltrate.

2. Fermentation of sweet whey by ethanologenic Escherichia coli.

3. Production of bacteriolytic activity in the oral cavity by nutritionally variant streptococci.

4. Yeast production from crude sweet whey.

5. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.

6. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

7. Enzyme-linked immunoelectrotransfer blot techniques (EITB) for studying the specificities of antigens and antibodies separated by gel electrophoresis.

8. Production of bacteriolytic enzymes as a tool for characterizing enterococci.

9. Biomass and lipids from lactose or whey by Trichosporon beigelii.

10. Kluyveromyces lactis rDNA as a target for multiple integration by homologous recombination.

1. Engineering Escherichia coli for soluble expression and single step purification of active human lysozyme.

2. SOD1, a new Kluyveromyces lactis helper gene for heterologous protein secretion.

3. Role of cultivation media in the development of yeast strains for large scale industrial use.

4. Purification and Characterization of Recombinant Human Lysozyme from Eggs of Transgenic Chickens.