Literature DB >> 10344504

Bioassay of biotin concentration with a Escherichia coli bio deletion mutant.

S Y Hwang1, V Su, L Farh, D Shiuan.   

Abstract

Biotin concentration was determined unequivocally with the E. coli bio mutant. The results demonstrate that this simple and efficient method can determine biotin concentration in the range of 10 pg to 50 ng/ml. The present method can also clearly distinguish biotin from its precursor and analog, dethiobiotin.

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Year:  1999        PMID: 10344504     DOI: 10.1016/s0165-022x(98)00012-8

Source DB:  PubMed          Journal:  J Biochem Biophys Methods        ISSN: 0165-022X


  2 in total

1.  Overexpression of biotin synthase and biotin ligase is required for efficient generation of sulfur-35 labeled biotin in E. coli.

Authors:  Teegan A Delli-Bovi; Maroya D Spalding; Sean T Prigge
Journal:  BMC Biotechnol       Date:  2010-10-11       Impact factor: 2.563

2.  A versatile Escherichia coli strain for identification of biotin transporters and for biotin quantification.

Authors:  Friedrich Finkenwirth; Franziska Kirsch; Thomas Eitinger
Journal:  Bioengineered       Date:  2013-11-05       Impact factor: 3.269

  2 in total

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