AIM: To investigate whether human herpesvirus 6 (HHV-6) can cause latent infection of liver tissue. METHODS: Peripheral blood and liver tissue were collected from 25 living related liver transplant recipients at the time of transplantation. An avidin-biotin complex peroxidase method was used to identify HHV-6 antigen in the liver tissue. A nested polymerase chain reaction (PCR) was used to detect HHV-6 DNA in the liver tissue and mononuclear cells. Variant of HHV-6 was determined by the presence of the Hind III site in a second PCR product. RESULTS: Immunohistochemical analysis for HHV-6 antigen was negative in all the liver specimens. HHV-6 DNA was not detected in liver tissue. Virus DNA was detected in peripheral blood mononuclear cells in nine of 25 recipients. All nine HHV-6 identified in the mononuclear cells were variant B. CONCLUSIONS: HHV-6 variant B latently infects mononuclear cells but not liver tissue.
AIM: To investigate whether human herpesvirus 6 (HHV-6) can cause latent infection of liver tissue. METHODS: Peripheral blood and liver tissue were collected from 25 living related liver transplant recipients at the time of transplantation. An avidin-biotin complex peroxidase method was used to identify HHV-6 antigen in the liver tissue. A nested polymerase chain reaction (PCR) was used to detect HHV-6 DNA in the liver tissue and mononuclear cells. Variant of HHV-6 was determined by the presence of the Hind III site in a second PCR product. RESULTS: Immunohistochemical analysis for HHV-6 antigen was negative in all the liver specimens. HHV-6 DNA was not detected in liver tissue. Virus DNA was detected in peripheral blood mononuclear cells in nine of 25 recipients. All nine HHV-6 identified in the mononuclear cells were variant B. CONCLUSIONS:HHV-6 variant B latently infects mononuclear cells but not liver tissue.
Authors: P E Pellett; D Sánchez-Martínez; G Dominguez; J B Black; E Anton; C Greenamoyer; T R Dambaugh Journal: Virology Date: 1993-08 Impact factor: 3.616
Authors: T Yoshikawa; S Suga; Y Asano; T Nakashima; T Yazaki; R Sobue; M Hirano; M Fukuda; S Kojima; T Matsuyama Journal: Blood Date: 1991-09-01 Impact factor: 22.113
Authors: S Z Salahuddin; D V Ablashi; P D Markham; S F Josephs; S Sturzenegger; M Kaplan; G Halligan; P Biberfeld; F Wong-Staal; B Kramarsky Journal: Science Date: 1986-10-31 Impact factor: 47.728
Authors: C A Schmidt; F Wilbron; K Weiss; V Brinkmann; H Oettle; R Lohmann; J M Langrehr; P Neuhaus; W Siegert Journal: Transplantation Date: 1996-02-27 Impact factor: 4.939