| Literature DB >> 10341417 |
M O Agaphonov1, P M Trushkina, J H Sohn, E S Choi, S K Rhee, M D Ter-Avanesyan.
Abstract
Plasmids with different selectable markers were constructed and used to transform the Hansenula polymorpha strain DL1. It was shown that, depending on the host mutant strain, the use of these plasmids enables rapid selection of transformants with plasmids integrated in low (1-2), moderate (6-9) or high (up to 100) copy numbers. The vectors and mutant described are potentially useful for the construction of efficient producers of heterologous proteins in H. polymorpha.Entities:
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Year: 1999 PMID： 10341417 DOI： 10.1002/(SICI)1097-0061(199905)15:7<541::AID-YEA392>3.0.CO;2-G
Source DB: PubMed Journal: Yeast ISSN： 0749-503X Impact factor: 3.239