OBJECTIVE: To investigate whether human preadipocytes possess a complete functional renin angiotensin system. MEASUREMENTS: Gene expression of angiotensinogen, renin, renin binding protein, angiotensin converting enzyme (ACE) and angiotensin II (ang II) receptor type 1 in human preadipocytes; ACE protein and ang II production of human adipose tissue stromal cells differentiated or not in primary culture. RESULTS: All genes mentioned above were found to be expressed in human preadipocytes. ACE was translated into protein as detected by western blot. Ang II was secreted both by undifferentiated preadipocytes and immature adipocytes, and its production was significantly elevated in differentiated cells. CONCLUSIONS: Preadipocytes from human adipose tissue express a functional renin angiotensin system (RAS).
OBJECTIVE: To investigate whether human preadipocytes possess a complete functional renin angiotensin system. MEASUREMENTS: Gene expression of angiotensinogen, renin, renin binding protein, angiotensin converting enzyme (ACE) and angiotensin II (ang II) receptor type 1 in human preadipocytes; ACE protein and ang II production of human adipose tissue stromal cells differentiated or not in primary culture. RESULTS: All genes mentioned above were found to be expressed in human preadipocytes. ACE was translated into protein as detected by western blot. Ang II was secreted both by undifferentiated preadipocytes and immature adipocytes, and its production was significantly elevated in differentiated cells. CONCLUSIONS: Preadipocytes from human adipose tissue express a functional renin angiotensin system (RAS).
Authors: Frederique Yiannikouris; Michael Karounos; Richard Charnigo; Victoria L English; Debra L Rateri; Alan Daugherty; Lisa A Cassis Journal: Am J Physiol Regul Integr Comp Physiol Date: 2011-11-09 Impact factor: 3.619
Authors: Jason D Fowler; Nathan D Johnson; Thomas A Haroldson; Joy A Brintnall; Julio E Herrera; Stephen A Katz; David A Bernlohr Journal: Am J Physiol Endocrinol Metab Date: 2009-03-17 Impact factor: 4.310
Authors: Claudia Agabiti-Rosei; Anna Paini; Carolina De Ciuceis; Sarah Withers; Adam Greenstein; Anthony M Heagerty; Damiano Rizzoni Journal: Curr Hypertens Rep Date: 2018-05-07 Impact factor: 5.369