Literature DB >> 1033145

Rainbow trout leukocyte culture: a simplified method.

H M Etlinger, H O Hodgins, J M Chiller.   

Abstract

The addition of 10% fetal bovine serum to Leibovitz's L-15 culture medium resulted in marked growth of peripheral blood leukocytes from rainbow trout, Salmo gairdneri. Culture medium without serum or with 20% homologous serum did not induce substantial growth. In contrast to what has been reported by others, oxygenation of the culture medium was found not to be required for excellent cell growth.

Entities:  

Mesh:

Substances:

Year:  1976        PMID: 1033145     DOI: 10.1007/BF02797445

Source DB:  PubMed          Journal:  In Vitro        ISSN: 0073-5655


  5 in total

1.  Preparation of chromosomes for light microscopy utilizing an agar fixation procedure.

Authors:  R E PACHA; D T KINGSBURY
Journal:  Proc Soc Exp Biol Med       Date:  1962-12

2.  Blastoid transformation of lymphocytes in vitro: the stimulatory effect of foetal calf serum.

Authors:  H J Woodliff; P Onesti
Journal:  Med J Aust       Date:  1968-06-22       Impact factor: 7.738

3.  Reaction of human lymphocytes in culture to components of the medium.

Authors:  G J Johnson; P S Russell
Journal:  Nature       Date:  1965-10-23       Impact factor: 49.962

4.  Trout leukocytes: growth in oxygenated cultures.

Authors:  J R Heckman; F W Allendorf; J E Wright
Journal:  Science       Date:  1971-07-16       Impact factor: 47.728

5.  In vitro stimulation of hamster lymphocytes with concanavalin A.

Authors:  S B Singh; S S Tevethia
Journal:  Infect Immun       Date:  1972-03       Impact factor: 3.441

  5 in total
  1 in total

1.  Mitogenesis of rainbow trout peripheral blood lymphocytes requires homologous plasma for optimal responsiveness.

Authors:  J DeKoning; S Kaattari
Journal:  In Vitro Cell Dev Biol       Date:  1991-05
  1 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.