OBJECTIVE: A one year study of the relation between cytomegalovirus (CMV) and human immunodeficiency virus (HIV) viral loads in a cohort of children with vertically acquired HIV-1 infection. DESIGN: Comparative analysis of viral load measurements for CMV and HIV-1 in peripheral blood leucocytes (PBLs) of individual children in relation to age and clinical staging. METHODS: Nested polymerase chain reaction (PCR) was used to measure HIV-1 proviral DNA and CMV genomic DNA in PBLs of 56 children. RESULTS: The CMV load was highest in 0-2 year old HIV positive children with stage C disease (range, 1-7143 copies/100 ng DNA; median, 125) and was significantly lower in older children. Although higher in young children, HIV-1 viral load did not show the same marked reduction with age that is seen with CMV. Over a one year period, testing of serial samples for both viruses in a subgroup of children revealed a discordant relation between viral loads for CMV and HIV-1. CONCLUSIONS: CMV viral load falls much faster than HIV viral load in dually infected children. Screening for clinical CMV disease is most likely to be of benefit in children under 2 years of age with stage C disease. In the few children studied, levels of CMV and HIV replication appear to be independent.
OBJECTIVE: A one year study of the relation between cytomegalovirus (CMV) and human immunodeficiency virus (HIV) viral loads in a cohort of children with vertically acquired HIV-1 infection. DESIGN: Comparative analysis of viral load measurements for CMV and HIV-1 in peripheral blood leucocytes (PBLs) of individual children in relation to age and clinical staging. METHODS: Nested polymerase chain reaction (PCR) was used to measure HIV-1 proviral DNA and CMV genomic DNA in PBLs of 56 children. RESULTS: The CMV load was highest in 0-2 year old HIV positive children with stage C disease (range, 1-7143 copies/100 ng DNA; median, 125) and was significantly lower in older children. Although higher in young children, HIV-1 viral load did not show the same marked reduction with age that is seen with CMV. Over a one year period, testing of serial samples for both viruses in a subgroup of children revealed a discordant relation between viral loads for CMV and HIV-1. CONCLUSIONS: CMV viral load falls much faster than HIV viral load in dually infected children. Screening for clinical CMV disease is most likely to be of benefit in children under 2 years of age with stage C disease. In the few children studied, levels of CMV and HIV replication appear to be independent.
Authors: B J Kitchen; H D Engler; V J Gill; D Marshall; S M Steinberg; P A Pizzo; B U Mueller Journal: Pediatr Infect Dis J Date: 1997-04 Impact factor: 2.129
Authors: Y S Boriskin; J C Booth; C M Corbishley; B P Madden; W J McKenna; A J Murday; H M Steel Journal: J Med Virol Date: 1996-09 Impact factor: 2.327
Authors: K McIntosh; A Shevitz; D Zaknun; J Kornegay; P Chatis; N Karthas; S K Burchett Journal: Pediatr Infect Dis J Date: 1996-12 Impact factor: 2.129
Authors: Jennifer A Slyker; Barbara L Lohman-Payne; Grace C John-Stewart; Elizabeth Maleche-Obimbo; Sandra Emery; Barbra Richardson; Tao Dong; Astrid Kn Iversen; Dorothy Mbori-Ngacha; Julie Overbaugh; Vincent C Emery; Sarah L Rowland-Jones Journal: AIDS Date: 2009-10-23 Impact factor: 4.177