BACKGROUND: In autoimmune bullous skin diseases, accumulation of neutrophils and/or eosinophils in the affected skin represents a characteristic feature. So far, however, the induction of this granulocyte infiltration has not been elucidated. OBJECTIVE: Regarding their biological effects, the chemokines interleukin 8 (IL-8) and RANTES (regulated upon activation normal T lymphocyte expressed and secreted) could play a role in this granulocyte accumulation. METHODS: Immunohistochemical examination of lesional skin from patients with bullous pemphigoid, pemphigus, dermatitis herpetiformis and linear IgA disease was performed using a set of different antibodies against IL-8 and RANTES. Additionally, blister fluids were screened for soluble RANTES peptide using an ELISA. RESULTS: No difference in chemokine expression was found in lesions of autoimmune bullous diseases compared to normal skin. CONCLUSION: In contrast to chronic inflammatory diseases like psoriasis and eczema, where keratinocyte IL-8 immunoreactivity was found to differ from normal skin, keratinocyte immunoreactivity is not altered in autoimmune bullous diseases.
BACKGROUND: In autoimmune bullous skin diseases, accumulation of neutrophils and/or eosinophils in the affected skin represents a characteristic feature. So far, however, the induction of this granulocyte infiltration has not been elucidated. OBJECTIVE: Regarding their biological effects, the chemokines interleukin 8 (IL-8) and RANTES (regulated upon activation normal T lymphocyte expressed and secreted) could play a role in this granulocyte accumulation. METHODS: Immunohistochemical examination of lesional skin from patients with bullous pemphigoid, pemphigus, dermatitis herpetiformis and linear IgA disease was performed using a set of different antibodies against IL-8 and RANTES. Additionally, blister fluids were screened for soluble RANTES peptide using an ELISA. RESULTS: No difference in chemokine expression was found in lesions of autoimmune bullous diseases compared to normal skin. CONCLUSION: In contrast to chronic inflammatory diseases like psoriasis and eczema, where keratinocyte IL-8 immunoreactivity was found to differ from normal skin, keratinocyte immunoreactivity is not altered in autoimmune bullous diseases.
Authors: Jenny S Breitenbach; Mark Rinnerthaler; Andrea Trost; Manuela Weber; Alfred Klausegger; Christina Gruber; Daniela Bruckner; Herbert A Reitsamer; Johann W Bauer; Michael Breitenbach Journal: Aging (Albany NY) Date: 2015-06 Impact factor: 5.682
Authors: Rodolfo Pessato Timóteo; Marcos Vinicius Silva; Djalma Alexandre Alves da Silva; Jonatas Da Silva Catarino; Fernando Henrique Canhoto Alves; Virmondes Rodrigues Júnior; Ana Maria Roselino; Helioswilton Sales-Campos; Carlo José Freire Oliveira Journal: Front Immunol Date: 2017-08-14 Impact factor: 7.561