W Ding1, H Shen, H Zhu. 1. Department of Environmental Health, Shanghai Medical University.
Abstract
OBJECTIVE: To study hepatotoxicity of Microsystis aeruginosa toxin (microcystin) and its prevention. METHODS: Cytotoxicity and the formation of reactive oxygen species (ROS) of microcystin extracted water bloom were studied in rat liver cells of primary culture. RESULTS: It was found that the release of lactic dehydrogenase (LDH) increased with a time- and dose-dependent pattern in the liver cells treated with microcystin, and a time- dependent formation of ROS also increased simultaneously. The antioxidant, desferrioxamine, could decrease the release of LDH and the formation of ROS in the liver cells treated with microcystin. CONCLUSION: Microcystin has obvious hepatotoxicity and the formation of ROS may play a certain role in its toxic effect on the rat liver cells.
OBJECTIVE: To study hepatotoxicity of Microsystis aeruginosa toxin (microcystin) and its prevention. METHODS:Cytotoxicity and the formation of reactive oxygen species (ROS) of microcystin extracted water bloom were studied in rat liver cells of primary culture. RESULTS: It was found that the release of lactic dehydrogenase (LDH) increased with a time- and dose-dependent pattern in the liver cells treated with microcystin, and a time- dependent formation of ROS also increased simultaneously. The antioxidant, desferrioxamine, could decrease the release of LDH and the formation of ROS in the liver cells treated with microcystin. CONCLUSION:Microcystin has obvious hepatotoxicity and the formation of ROS may play a certain role in its toxic effect on the rat liver cells.