Literature DB >> 10322036

Regulation of beta-galactosidase expression in Bacillus megaterium DSM319 by a XylS/AraC-type transcriptional activator.

J Strey1, K D Wittchen, F Meinhardt.   

Abstract

The beta-galactosidase-encoding bgaM gene of Bacillus megaterium DSM319 and the divergently orientated bgaR operon were isolated and sequenced. Both traits are subject to catabolite repression. A set of single-gene replacement mutants was generated and used to analyze gene function. BgaR was found to be a XylS/AraC-type positive transcriptional regulator of bgaM; a potential regulator binding site overlaps the bgaM promoter. A mechanism for regulation of beta-galactosidase expression in B. megaterium is proposed.

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Year:  1999        PMID： 10322036      PMCID： PMC93790     

Source DB:  PubMed          Journal:  J Bacteriol        ISSN： 0021-9193            Impact factor:   3.490


  26 in total

1.  Properties and induction of beta-galactosidase in Bacillus megaterium.

Authors:  O E LANDMAN
Journal:  Biochim Biophys Acta       Date:  1957-03

2.  Site-directed mutagenesis of a catabolite repression operator sequence in Bacillus subtilis.

Authors:  M J Weickert; G H Chambliss
Journal:  Proc Natl Acad Sci U S A       Date:  1990-08       Impact factor: 11.205

3.  The XylS/AraC family of regulators.

Authors:  M T Gallegos; C Michán; J L Ramos
Journal:  Nucleic Acids Res       Date:  1993-02-25       Impact factor: 16.971

4.  Lambda replacement vectors carrying polylinker sequences.

Authors:  A M Frischauf; H Lehrach; A Poustka; N Murray
Journal:  J Mol Biol       Date:  1983-11-15       Impact factor: 5.469

5.  Isolation and characterization of the lacA gene encoding beta-galactosidase in Bacillus subtilis and a regulator gene, lacR.

Authors:  R A Daniel; J Haiech; F Denizot; J Errington
Journal:  J Bacteriol       Date:  1997-09       Impact factor: 3.490

6.  Structure of the beta-1,3-1,4-glucanase gene of Bacillus macerans: homologies to other beta-glucanases.

Authors:  R Borriss; K Buettner; P Maentsaelae
Journal:  Mol Gen Genet       Date:  1990-07

7.  Catabolite repression of alpha-amylase gene expression in Bacillus subtilis involves a trans-acting gene product homologous to the Escherichia coli lacl and galR repressors.

Authors:  T M Henkin; F J Grundy; W L Nicholson; G H Chambliss
Journal:  Mol Microbiol       Date:  1991-03       Impact factor: 3.501

8.  Cloning and sequencing of the leu C and npr M genes and a putative spo IV gene from Bacillus megaterium DSM319.

Authors:  F Meinhardt; M Busskamp; K D Wittchen
Journal:  Appl Microbiol Biotechnol       Date:  1994-05       Impact factor: 4.813

9.  Expression and nucleotide sequence of the Clostridium acetobutylicum beta-galactosidase gene cloned in Escherichia coli.

Authors:  K R Hancock; E Rockman; C A Young; L Pearce; I S Maddox; D B Scott
Journal:  J Bacteriol       Date:  1991-05       Impact factor: 3.490

10.  Analysis of the lacZ sequences from two Streptococcus thermophilus strains: comparison with the Escherichia coli and Lactobacillus bulgaricus beta-galactosidase sequences.

Authors:  C J Schroeder; C Robert; G Lenzen; L L McKay; A Mercenier
Journal:  J Gen Microbiol       Date:  1991-02
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  1 in total

1.  Identification of a lactose-responsive element upstream of the promoter of Bacillus megaterium beta-galactosidase-encoding gene mbgA.

Authors:  Jen-Ming Li; Chih-Yung Chiou; Tian-Ren Lee; Yuan-Shou Chen; Gwo-Chyuan Shaw
Journal:  Curr Microbiol       Date:  2005-06-16       Impact factor: 2.188
  1 in total

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