Effect of environmental factors and tissue culture methodology in producing and studying cultured cardiac cells.

Improvement in the method of heart cell cultures is described and justified in relation to environmental factors. The validity of such cardiac cell culture for cardiac research is discussed in light of particular cellular activities: differentiation of lipoprotein lipase, myoglobin biosynthesis, glucose and fatty acid metabolism pleiotypic responses (rotein, RNA, and DNA biosyntheses, substrates transports) to serum stimulation, and the architectonic growth of muscle and nonmuscle cells.