Isolation and chromosomal assignment of a human gene encoding protein inhibitor of activated STAT3 (PIAS3).

Mouse PIAS3 (protein inhibitor of activated STAT3) is a specific inhibitor of STAT3 that downregulates its signaling pathway. Here we report the isolation and chromosome mapping of the human PIAS3 gene. Human PIAS3 cDNA encoded a predicted protein of 619 aa which has 83% overall amino acid identity to the mouse counterpart. Based on polymerase chain reaction assisted analysis of a human/rodent mono-chromosomal hybrid cell panel and a radiation hybrid mapping panel, the human PIAS3 gene was mapped to the chromosome 1q21 region. Mapping of a crucial gene in modulating the STAT3 signaling pathway may provide new clues to the understanding of malignancies or genetic disorders caused by this chromosome region.