Literature DB >> 1031679

The large-scale cultivation of diploid cell strains in microcarrier culture. Improvement of microcarriers.

A L van Wezel.   

Abstract

The use of diploid cell strains for virus vaccine production is mostly retarded by the inefficient culture techniques used for the propagation of these cells. The microcarrier culture system might be a good solution for this problem. DEAE-gels appear to be the most satisfactory microcarrier. The best results are obtained by using DEAE-gels with a rather low anionic exchange capacity or at higher capacity after coating with polymers.

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Year:  1976        PMID: 1031679

Source DB:  PubMed          Journal:  Dev Biol Stand        ISSN: 0301-5149


  6 in total

1.  Microcarrier technology, present status and perspective.

Authors:  C A van der Velden-de Groot
Journal:  Cytotechnology       Date:  1995-01       Impact factor: 2.058

2.  Exosomes Produced from 3D Cultures of MSCs by Tangential Flow Filtration Show Higher Yield and Improved Activity.

Authors:  Reka Agnes Haraszti; Rachael Miller; Matteo Stoppato; Yves Y Sere; Andrew Coles; Marie-Cecile Didiot; Rachel Wollacott; Ellen Sapp; Michelle L Dubuke; Xuni Li; Scott A Shaffer; Marian DiFiglia; Yang Wang; Neil Aronin; Anastasia Khvorova
Journal:  Mol Ther       Date:  2018-09-22       Impact factor: 11.454

3.  Pure gelatin microcarriers: synthesis and use in cell attachment and growth of fibroblast and endothelial cells.

Authors:  K W Wissemann; B S Jacobson
Journal:  In Vitro Cell Dev Biol       Date:  1985-07

4.  Use of biocarrier beads and flow cytometry for single-cell studies of fibronectin gene regulation in dibutyryl cyclic AMP reverse transformed CHO-K1 cells.

Authors:  J M Sterner; J F Leary
Journal:  Cell Biophys       Date:  1989-12

5.  Cell growth optimization in microcarrier culture.

Authors:  B Mered; P Albrecht; H E Hopps
Journal:  In Vitro       Date:  1980-10

6.  Culture of respiratory syncytial virus infected diploid bovine nasal mucosa cells on cytodex 3 microcarriers.

Authors:  A J Hayle
Journal:  Arch Virol       Date:  1986       Impact factor: 2.574

  6 in total

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