Literature DB >> 1030711

Ectogalactosyltransferase studies in fibroblasts and concanavalin A-stimulated lymphocytes.

L M Patt, R O Endres, D O Lucas, W J Grimes.   

Abstract

In this communication, we have demonstrated that hydrolysis of the nucleotide sugar can cause errors in the detection of an ectoglycosyltransferase. Spleen cell suspensions can incorporate radioactivity when incubated with labeled UDP-galactose, but all the activity is due to decomposition of the nucleotide sugar and uptake of the free sugar. The fibroblast cell lines can incroporate carbohydrate directly from UDP-galactose. Several criteria are presented with can be used to demonstrate that a nucleotide sugar is the direct carbohydrate donor.

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Year:  1976        PMID： 1030711      PMCID： PMC2109644          DOI： 10.1083/jcb.68.3.799

Source DB:  PubMed          Journal:  J Cell Biol        ISSN： 0021-9525            Impact factor:   10.539


  12 in total

1.  Cell surface galactosyltransferase and lectin agglutination of thymus and spleen lymphocytes.

Authors:  J T Lamont; J L Perrotto; M M Weiser; K J Isselbacher
Journal:  Proc Natl Acad Sci U S A       Date:  1974-09       Impact factor: 11.205

2.  Nucleotide pyrophosphatase, a sialoglycoprotein located on the hepatocyte surface.

Authors:  W H Evans
Journal:  Nature       Date:  1974-08-02       Impact factor: 49.962

3.  Cell surface glycolipid and glycoprotein glycosyltransferases of normal and transformed cells.

Authors:  L M Patt; W J Grimes
Journal:  J Biol Chem       Date:  1974-07-10       Impact factor: 5.157

4.  Permeation of glucose by simple and facilitated diffusion by Novikoff rat hepatoma cells in suspension culture and its relationship to glucose metabolism.

Authors:  E D Renner; P G Plagemann; R W Bernlohr
Journal:  J Biol Chem       Date:  1972-09-25       Impact factor: 5.157

5.  Hexose transport in normal and in Rous sarcoma virus-transformed cells.

Authors:  M J Weber
Journal:  J Biol Chem       Date:  1973-05-10       Impact factor: 5.157

6.  Folic acid effects on glycoprotein-galactosyltransferase: a re-assessment.

Authors:  L M Geren; K E Ebner
Journal:  Biochem Biophys Res Commun       Date:  1974-07-10       Impact factor: 3.575

Review 7.  A molecular model for cell interactions.

Authors:  S Roth
Journal:  Q Rev Biol       Date:  1973-12       Impact factor: 4.875

8.  Differentiation between intracellular and cell surface glycosyl transferases: galactosyl transferase activity in intact cells and in cell homogenate.

Authors:  W Deppert; H Werchau; G Walter
Journal:  Proc Natl Acad Sci U S A       Date:  1974-08       Impact factor: 11.205

9.  Sugar transport in chick embryo fibroblasts. I. A functional change in the plasma membrane associated with the rate of cell growth.

Authors:  R F Kletzien; J F Perdue
Journal:  J Biol Chem       Date:  1974-06-10       Impact factor: 5.157

Review 10.  Plasma membranes of mammalian cells: a review of methods for their characterization and isolation.

Authors:  J W DePierre; M L Karnovsky
Journal:  J Cell Biol       Date:  1973-02       Impact factor: 10.539
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  2 in total

Review 1.  The receptor function of galactosyltransferase during cellular interactions.

Authors:  B D Shur
Journal:  Mol Cell Biochem       Date:  1984       Impact factor: 3.396

2.  The site of incorporation of sialic acid residues into glycoproteins and the subsequent fates of these molecules in various rat and mouse cell types as shown by radioautography after injection of [3H]N-acetylmannosamine. I. Observations in hepatocytes.

Authors:  G Bennett; D O'Shaughnessy
Journal:  J Cell Biol       Date:  1981-01       Impact factor: 10.539
  2 in total

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