| Literature DB >> 10233775 |
D R Bielenberg1, M F McCarty, C D Bucana, S H Yuspa, D Morgan, J M Arbeit, L M Ellis, K R Cleary, I J Fidler.
Abstract
The cytokine interferon-beta is a regulator of cell replication and function, including invasion and induction of angiogenesis. The goal of this study was to determine whether the expression of interferon-beta by cells in the epidermis correlated with terminal differentiation. In situ hybridization analysis and immunohistochemical staining of formalin-fixed, paraffin-embedded specimens of normal human and murine epidermis and human and murine skin tumors of epithelial origin revealed that only differentiated, nondividing cells of the epidermis expressed interferon-beta protein. Keratinocyte cultures established from the epidermis of 3 d old mice were maintained under conditions permitting continuous cell division or induction of differentiation. Continuously dividing cells did not produce interferon-beta whereas nondividing differentiated cells expressing keratin 1 did. Growth-arrested, undifferentiated keratinocytes also expressed interferon-beta protein. Neutralizing interferon-beta in the culture medium inhibited differentiation, but the addition of exogenous interferon-beta did not stimulate differentiation. These data indicate that interferon-beta is produced by growth-arrested, terminally differentiated keratinocytes.Entities:
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Year: 1999 PMID： 10233775 DOI： 10.1046/j.1523-1747.1999.00566.x
Source DB: PubMed Journal: J Invest Dermatol ISSN： 0022-202X Impact factor: 8.551