J C Reinhardt1, X Cui, W E Roudebush. 1. Department of Obstetrics and Gynecology, Medical University of South Carolina, Charleston 29425, USA.
Abstract
OBJECTIVE: To determine the presence of the platelet-activating factor (PAF) receptor on human spermatozoa. DESIGN: Prospective analysis. SETTING: University-based reproductive biology laboratory. PATIENT(S): Spermatozoa obtained from men undergoing routine semen analysis. INTERVENTION(S): Spermatozoa (n = 10) were exposed to PAF, sheep anti-PAF antibody, and fluorescein isothiocyanate-conjugated rabbit antisheep antibody, and then evaluated by fluorescent microscopy. MAIN OUTCOME MEASURE(S): Assessment of fluorescent intensity at four locations on the spermatozoa (tail, midpiece, proximal head, and acrosome region). RESULT(S): Immunofluorescence demonstrated the presence of the PAF receptor on human spermatozoa. The PAF receptor was most prevalent at two sites on the spermatozoa: the midpiece and the proximal head. CONCLUSION(S): The PAF receptor is present on human spermatozoa. Platelet-activating factor may affect the motility of spermatozoa through a receptor-mediated mechanism at the midpiece and/or proximal head.
OBJECTIVE: To determine the presence of the platelet-activating factor (PAF) receptor on human spermatozoa. DESIGN: Prospective analysis. SETTING: University-based reproductive biology laboratory. PATIENT(S): Spermatozoa obtained from men undergoing routine semen analysis. INTERVENTION(S): Spermatozoa (n = 10) were exposed to PAF, sheep anti-PAF antibody, and fluorescein isothiocyanate-conjugated rabbit antisheep antibody, and then evaluated by fluorescent microscopy. MAIN OUTCOME MEASURE(S): Assessment of fluorescent intensity at four locations on the spermatozoa (tail, midpiece, proximal head, and acrosome region). RESULT(S): Immunofluorescence demonstrated the presence of the PAF receptor on human spermatozoa. The PAF receptor was most prevalent at two sites on the spermatozoa: the midpiece and the proximal head. CONCLUSION(S): The PAF receptor is present on human spermatozoa. Platelet-activating factor may affect the motility of spermatozoa through a receptor-mediated mechanism at the midpiece and/or proximal head.
Authors: C Briton-Jones; Q S Yeung; G C Tjer; T T Chiu; L P Cheung; S F Yim; I H Lok; C Haines Journal: J Assist Reprod Genet Date: 2001-03 Impact factor: 3.412
Authors: Andrew A Toledo; Dorothy Mitchell-Leef; Carlene W Elsner; Scott M Slayden; William E Roudebush Journal: J Assist Reprod Genet Date: 2003-05 Impact factor: 3.412