Literature DB >> 10231022

Co-activation of Gi and Gq proteins exerts synergistic effect on human platelet aggregation through activation of phospholipase C and Ca2+ signalling pathways.

B H Shah1, A Siddiqui, K A Qureshi, M Khan, S Rafi, V A Ujan, M Y Yakoob, Y Yaqub, H Rasheed, S A Saeed.   

Abstract

Our previous studies have shown that subthreshold concentrations of two platelet agonists exert synergistic effects on platelet aggregation. Here we studied the mechanism of synergistic interaction of 5-hydroxytryptamine (5-HT) and epinephrine mediated platelet aggregation. We show that 5-HT had no or little effect on aggregation but it did potentiate the aggregation response of epinephrine. The synergistic interaction of 5-HT (1-5 microM) and epinephrine (0.5-2 microM) was inhibited by alpha2-adrenoceptor blocker (yohimbine; IC50= 0.4 microM), calcium channel blockers (verapamil and diltiazem with IC50 of 10 and 48 mM, respectively), PLC inhibitor (U73122; IC50=6 microM) and nitric oxide (NO) donor, SNAP (IC50=1.6 microM)). The data suggest that synergistic effects of platelet agonists are receptor-mediated and occur through multiple signalling pathways including the activation PLC/Ca2+ signalling cascades.

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Year:  1999        PMID： 10231022     DOI： 10.1038/emm.1999.7

Source DB:  PubMed          Journal:  Exp Mol Med        ISSN： 1226-3613            Impact factor:   8.718


  8 in total

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2.  Enhanced brain stem 5HT₂A receptor function under neonatal hypoxic insult: role of glucose, oxygen, and epinephrine resuscitation.

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3.  Synergistic activation of phospholipase C-beta3 by Galpha(q) and Gbetagamma describes a simple two-state coincidence detector.

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Journal:  Curr Biol       Date:  2010-06-24       Impact factor: 10.834

4.  The orphan receptor GPR139 signals via Gq/11 to oppose opioid effects.

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Review 5.  Mechanisms of cross-talk between G-protein-coupled receptors resulting in enhanced release of intracellular Ca2+.

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Journal:  J Cell Sci       Date:  2016-08-15       Impact factor: 5.285

7.  Investigation of cyclooxygenase and signaling pathways involved in human platelet aggregation mediated by synergistic interaction of various agonists.

Authors:  Nadia Khan; Ahsana Dar Farooq; Bassem Sadek
Journal:  Drug Des Devel Ther       Date:  2015-07-06       Impact factor: 4.162

8.  The antithrombotic and fibrinolytic effect of natto in hypercholesterolemia rats.

Authors:  Kum-Ju Park; Jung Il Kang; Tae-Seok Kim; Ik-Hyun Yeo
Journal:  Prev Nutr Food Sci       Date:  2012-03
  8 in total

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