PURPOSE: Sialyl Lewis X (sLex), Lewis X (Lex), and N-acetyllactosamine are carbohydrate chains of neolactoglycoconjugates which are expressed by specific cell types and are important in the functioning of cells within an organism. This study attempts to determine the expression of these glycoconjugates on the conjunctiva, cornea, and trabecular meshwork (TM) of both normal and glaucomatous eyes. METHODS: Frozen anterior segment sections of both normal and glaucomatous human cadaver eyes, as well as rabbit eyes, were stained with a panel of monoclonal antibodies (mAbs) to neolactoglycoconjugates using an Avidin Biotin Peroxidase Complex/Alkaline Phosphatase staining method. RESULTS: SLex characteristically stained both human conjunctival and corneal epithelia in normal (n=5) and glaucomatous (n=5) sections. SLex stained corneal and conjunctival epithelia of glaucomatous eyes much more intensely than normal eyes. Rabbit cornea sections stained for sLex, Lex, and N-acetyllactosamine. However, human cornea only consistently stained with sLex. Normal and glaucomatous human TM sections did not stain for sLex, Lex, or N-acetyllactosamine. CONCLUSIONS: The expression of glycoconjugates with sLex side chains appears to be upregulated in the conjunctival and corneal epithelia of glaucomatous eyes. Distinct species specific differences were noted in Lex and N-acetyllactosamine staining patterns in rabbit and human corneal epithelia.
PURPOSE: Sialyl Lewis X (sLex), Lewis X (Lex), and N-acetyllactosamine are carbohydrate chains of neolactoglycoconjugates which are expressed by specific cell types and are important in the functioning of cells within an organism. This study attempts to determine the expression of these glycoconjugates on the conjunctiva, cornea, and trabecular meshwork (TM) of both normal and glaucomatous eyes. METHODS: Frozen anterior segment sections of both normal and glaucomatoushuman cadaver eyes, as well as rabbit eyes, were stained with a panel of monoclonal antibodies (mAbs) to neolactoglycoconjugates using an Avidin Biotin Peroxidase Complex/Alkaline Phosphatase staining method. RESULTS: SLex characteristically stained both human conjunctival and corneal epithelia in normal (n=5) and glaucomatous (n=5) sections. SLex stained corneal and conjunctival epithelia of glaucomatous eyes much more intensely than normal eyes. Rabbit cornea sections stained for sLex, Lex, and N-acetyllactosamine. However, human cornea only consistently stained with sLex. Normal and glaucomatoushuman TM sections did not stain for sLex, Lex, or N-acetyllactosamine. CONCLUSIONS: The expression of glycoconjugates with sLex side chains appears to be upregulated in the conjunctival and corneal epithelia of glaucomatous eyes. Distinct species specific differences were noted in Lex and N-acetyllactosamine staining patterns in rabbit and humancorneal epithelia.
Authors: Michael C W Chan; Renee W Y Chan; Wendy C L Yu; Carol C C Ho; Kit M Yuen; Joanne H M Fong; Lynsia L S Tang; Wico W Lai; Amy C Y Lo; W H Chui; Alan D L Sihoe; Dora L W Kwong; David S H Wong; George S W Tsao; Leo L M Poon; Yi Guan; John M Nicholls; Joseph S M Peiris Journal: Am J Pathol Date: 2010-01-28 Impact factor: 4.307
Authors: M Reza Nokhbeh; Samir Hazra; David A Alexander; Ahmar Khan; Morgan McAllister; Erik J Suuronen; May Griffith; Kenneth Dimock Journal: J Virol Date: 2005-06 Impact factor: 5.103
Authors: Jessica A Belser; Ola Blixt; Li-Mei Chen; Claudia Pappas; Taronna R Maines; Neal Van Hoeven; Ruben Donis; Julia Busch; Ryan McBride; James C Paulson; Jacqueline M Katz; Terrence M Tumpey Journal: Proc Natl Acad Sci U S A Date: 2008-05-27 Impact factor: 11.205
Authors: Jessica A Belser; Kortney M Gustin; Taronna R Maines; Mary J Pantin-Jackwood; Jacqueline M Katz; Terrence M Tumpey Journal: PLoS Pathog Date: 2012-03-01 Impact factor: 6.823