Literature DB >> 10222665

[Investigation of a new p24 antigen detection system by the chemiluminescence-enzyme-immuno-assay].

A Sakai1, Y Hirabayashi, S Aizawa, M Tanaka, S Ida, S Oka.   

Abstract

We investigated a new p24 antigen detection system by the chemiluminescence-enzyme-immuno-assay (CLEIA) and compared it with the conventional antigen-kits (A; Abbott, B; Coulter). The detection limit of CLEIA, A, and B were 4.3 pg/ml, 11.3 pg/ml, and 5.6 pg/ml, respectively. When the patient's sera and panel sera were examined by the three method, compatible results with the detection limit were obtained. CLEIA can be completed within 35 min, and is performed by an automatic system, which can treat many samples at one time. Therefore, this system is more suitable for screening of donor blood because of the shortening of the window period.

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Year:  1999        PMID： 10222665     DOI： 10.11150/kansenshogakuzasshi1970.73.205

Source DB:  PubMed          Journal:  Kansenshogaku Zasshi        ISSN： 0387-5911


  5 in total

1.  Prevention of HIV-1 infection in human peripheral blood mononuclear cells by specific RNA interference.

Authors:  Wee-Sung Park; Naoko Miyano-Kurosaki; Masaaki Hayafune; Emiko Nakajima; Tetsuo Matsuzaki; Fumiyuki Shimada; Hiroshi Takaku
Journal:  Nucleic Acids Res       Date:  2002-11-15       Impact factor: 16.971

2.  HIV-1 RT-dependent DNAzyme expression inhibits HIV-1 replication without the emergence of escape viruses.

Authors:  Ryuichi Sugiyama; Masaaki Hayafune; Yuichiro Habu; Norio Yamamoto; Hiroshi Takaku
Journal:  Nucleic Acids Res       Date:  2010-09-09       Impact factor: 16.971

3.  Inhibition of HIV-1 gene expression by retroviral vector-mediated small-guide RNAs that direct specific RNA cleavage by tRNase ZL.

Authors:  Yuichiro Habu; Naoko Miyano-Kurosaki; Michiko Kitano; Yumihiko Endo; Masakazu Yukita; Shigeru Ohira; Hiroaki Takaku; Masayuki Nashimoto; Hiroshi Takaku
Journal:  Nucleic Acids Res       Date:  2005-01-12       Impact factor: 16.971

4.  Long-term transgene expression and inhibition of HIV-1 replication by a Cre/loxP-EBNA-1/oriP HIV-1-dependent ribozyme vector: Applications for HIV-1 gene therapy.

Authors:  Takashi Nagawa; Yuichiro Habu; Norihiko Matsumoto; Naoko Miyano-Kurosaki; Hiroshi Takaku
Journal:  J RNAi Gene Silencing       Date:  2006-01-13

5.  Short-hairpin RNAs synthesized by T7 phage polymerase do not induce interferon.

Authors:  Takuma Gondai; Kazuya Yamaguchi; Naoko Miyano-Kurosaki; Yuichirou Habu; Hiroshi Takaku
Journal:  Nucleic Acids Res       Date:  2008-01-21       Impact factor: 16.971
  5 in total

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