Literature DB >> 10220893

Functional analysis of the hemK gene product involvement in protoporphyrinogen oxidase activity in yeast.

L Le Guen1, R Santos, J M Camadro.   

Abstract

The Escherichia coli hemK gene has been described as being involved in protoporphyrinogen oxidase activity; however, there is no biochemical evidence for this. In the context of characterizing the mechanisms of protoporphyrinogen oxidation in the yeast Saccharomyces cerevisiae, we investigated the yeast homolog of HemK, which is encoded by the ORF YNL063w, to find out whether it has any protoporphyrinogen oxidase activity and/or whether it modulates protoporphyrinogen oxidase activity. Phenotype analysis and enzyme activity measurements indicated that the yeast HemK homolog is not involved in protoporphyrinogen oxidase activity. Complementation assays in which the yeast HemK homolog is overproduced do not restore wild-type phenotypes in a yeast strain with deficient protoporphyrinogen oxidase activity. Protein sequence analysis of HemK-related proteins revealed consensus motif for S-adenosyl-methionine-dependent methyltransferase.

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Year:  1999        PMID： 10220893     DOI： 10.1111/j.1574-6968.1999.tb13499.x

Source DB:  PubMed          Journal:  FEMS Microbiol Lett        ISSN： 0378-1097            Impact factor:   2.742


  8 in total

Review 1.  The methylator meets the terminator.

Authors:  Steven Clarke
Journal:  Proc Natl Acad Sci U S A       Date:  2002-02-05       Impact factor: 11.205

2.  HemK, a class of protein methyl transferase with similarity to DNA methyl transferases, methylates polypeptide chain release factors, and hemK knockout induces defects in translational termination.

Authors:  Kenji Nakahigashi; Naoko Kubo; Shin-ichiro Narita; Takeshi Shimaoka; Simon Goto; Taku Oshima; Hirotada Mori; Maki Maeda; Chieko Wada; Hachiro Inokuchi
Journal:  Proc Natl Acad Sci U S A       Date:  2002-01-22       Impact factor: 11.205

3.  RNAi-mediated knock-down of gene mN6A1 reduces cell proliferation and decreases protein translation.

Authors:  Yongbo Liu; Dongsong Nie; Yuelong Huang; Guangxiu Lu
Journal:  Mol Biol Rep       Date:  2008-04-04       Impact factor: 2.316

4.  The hemK gene in Escherichia coli encodes the N(5)-glutamine methyltransferase that modifies peptide release factors.

Authors:  Valérie Heurgué-Hamard; Stéphanie Champ; Ake Engström; Måns Ehrenberg; Richard H Buckingham
Journal:  EMBO J       Date:  2002-02-15       Impact factor: 11.598

5.  Borrelia Host Adaptation Protein (BadP) Is Required for the Colonization of a Mammalian Host by the Agent of Lyme Disease.

Authors:  Trever C Smith; Sarah M Helm; Yue Chen; Ying-Han Lin; S L Rajasekhar Karna; J Seshu
Journal:  Infect Immun       Date:  2018-06-21       Impact factor: 3.441

6.  Structural characterization and comparative phylogenetic analysis of Escherichia coli HemK, a protein (N5)-glutamine methyltransferase.

Authors:  Zhe Yang; Lance Shipman; Meng Zhang; Brian P Anton; Richard J Roberts; Xiaodong Cheng
Journal:  J Mol Biol       Date:  2004-07-16       Impact factor: 5.469

7.  Substrate Specificity of the HEMK2 Protein Glutamine Methyltransferase and Identification of Novel Substrates.

Authors:  Denis Kusevic; Srikanth Kudithipudi; Albert Jeltsch
Journal:  J Biol Chem       Date:  2016-01-21       Impact factor: 5.157

8.  Cloning and primarily function study of two novel putative N5-glutamine methyltransferase (Hemk) splice variants from mouse stem cells.

Authors:  Dong-Song Nie; Yong-Bo Liu; Guang-Xiu Lu
Journal:  Mol Biol Rep       Date:  2008-12-30       Impact factor: 2.316
  8 in total

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