Literature DB >> 10217401

RNA helicase activity of Semliki Forest virus replicase protein NSP2.

M Gomez de Cedrón1, N Ehsani, M L Mikkola, J A García, L Kääriäinen.   

Abstract

Semliki Forest virus replicase protein nsP2 shares sequence homology with several putative NTPases and RNA helicases. NsP2 has RNA-dependent NTPase activity. Here we expressed polyhistidine-tagged nsP2 in Escherichia coli, purified it by metal-affinity chromatography, and used it in RNA helicase assays. RNA helicase CI of plum pox potyvirus was used as a positive control. Unwinding of alpha-32P-labelled partially double-stranded RNA required nsP2, Mg2+ and NTPs. NsP2 with a mutation, K192N, in the NTP-binding sequence GVPGSGK192SA could not unwind dsRNA and had no NTPase activity. This is the first demonstration of RNA helicase activity within the large alphavirus superfamily.

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Year:  1999        PMID： 10217401     DOI： 10.1016/s0014-5793(99)00321-x

Source DB:  PubMed          Journal:  FEBS Lett        ISSN： 0014-5793            Impact factor:   4.124


  84 in total

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3.  Coordinate replication of alfalfa mosaic virus RNAs 1 and 2 involves cis- and trans-acting functions of the encoded helicase-like and polymerase-like domains.

Authors:  A Corina Vlot; Sebastiaan M Laros; John F Bol
Journal:  J Virol       Date:  2003-10       Impact factor: 5.103

4.  Identification of the amino acid sequence in Sindbis virus nsP4 that binds to the promoter for the synthesis of the subgenomic RNA.

Authors:  Mei-Ling Li; Victor Stollar
Journal:  Proc Natl Acad Sci U S A       Date:  2004-06-14       Impact factor: 11.205

5.  Changes of the secondary structure of the 5' end of the Sindbis virus genome inhibit virus growth in mosquito cells and lead to accumulation of adaptive mutations.

Authors:  Rafik Fayzulin; Ilya Frolov
Journal:  J Virol       Date:  2004-05       Impact factor: 5.103

6.  Purification, crystallization and X-ray diffraction analysis of the C-terminal protease domain of Venezuelan equine encephalitis virus nsP2.

Authors:  Andrew T Russo; Stanley J Watowich
Journal:  Acta Crystallogr Sect F Struct Biol Cryst Commun       Date:  2006-05-05

7.  Identification of mutations causing temperature-sensitive defects in Semliki Forest virus RNA synthesis.

Authors:  Valeria Lulla; Andres Merits; Peter Sarin; Leevi Kääriäinen; Sirkka Keränen; Tero Ahola
Journal:  J Virol       Date:  2006-03       Impact factor: 5.103

8.  Identification of thieno[3,2-b]pyrrole derivatives as novel small molecule inhibitors of neurotropic alphaviruses.

Authors:  Weiping Peng; Daniel C Peltier; Martha J Larsen; Paul D Kirchhoff; Scott D Larsen; Richard R Neubig; David J Miller
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Review 9.  Helicases as antiviral drug targets.

Authors:  David N Frick
Journal:  Drug News Perspect       Date:  2003 Jul-Aug

10.  A mutant of Sindbis virus which is able to replicate in cells with reduced CTP makes a replicase/transcriptase with a decreased Km for CTP.

Authors:  Mei-Ling Li; Yen-Huei Lin; H Anne Simmonds; Victor Stollar
Journal:  J Virol       Date:  2004-09       Impact factor: 5.103
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