| Literature DB >> 10216290 |
J Ellis1, D J Campopiano, T Izard.
Abstract
Chloramphenicol 3-O-phosphotransferase (CPT) from Streptomyces venezuelae ISP5230, a novel chloramphenicol-inactivating kinase, has been overexpressed and purified using Escherichia coli as the heterologous host. Crystals of CPT in complex with its substrate chloramphenicol (Cm) were obtained which were suitable for X-ray diffraction. The crystals belong to the cubic space group I4132 with unit-cell dimension a = 200.0 A. The initial CPT crystals diffracted to 3.5 A and the diffraction was improved significantly upon adding acetonitrile and Cm to the crystallization drop. The CPT-Cm crystals diffract to at least 2.8 A resolution.Entities:
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Year: 1999 PMID： 10216290 DOI： 10.1107/s0907444999003029
Source DB: PubMed Journal: Acta Crystallogr D Biol Crystallogr ISSN： 0907-4449