Literature DB >> 10215579

Purification and characterization of an acetyl xylan esterase from Bacillus pumilus.

G Degrassi1, B C Okeke, C V Bruschi, V Venturi.   

Abstract

Bacillus pumilus PS213 was found to be able to release acetate from acetylated xylan. The enzyme catalyzing this reaction has been purified to homogeneity and characterized. The enzyme was secreted, and its production was induced by corncob powder and xylan. Its molecular mass, as determined by gel filtration, is 190 kDa, while sodium dodecyl sulfate-polyacrylamide gel electrophoresis showed a single band of 40 kDa. The isoelectric point was found to be 4.8, and the enzyme activity was optimal at 55 degrees C and pH 8.0. The activity was inhibited by most of the metal ions, while no enhancement was observed. The Michaelis contant (Km) and Vmax for alpha-naphthyl acetate were 1.54 mM and 360 micromol min-1 mg of protein-1, respectively.

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Year:  1998        PMID： 10215579      PMCID： PMC106121     

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN： 0099-2240            Impact factor:   4.792


  14 in total

1.  Ferulic Acid Esterase Activity from Schizophyllum commune.

Authors:  C R Mackenzie; D Bilous
Journal:  Appl Environ Microbiol       Date:  1988-05       Impact factor: 4.792

2.  Purification and characterization of two acetyl xylan esterases from Penicillium purpurogenum.

Authors:  L Egaña; R Gutiérrez; V Caputo; A Peirano; J Steiner; J Eyzaguirre
Journal:  Biotechnol Appl Biochem       Date:  1996-08       Impact factor: 2.431

3.  Structure and expression of genes coding for xylan-degrading enzymes of Bacillus pumilus.

Authors:  H Moriyama; E Fukusaki; J Cabrera Crespo; A Shinmyo; H Okada
Journal:  Eur J Biochem       Date:  1987-08-03

4.  Purification and characterization of an acetyl xylan esterase produced by Streptomyces lividans.

Authors:  C Dupont; N Daigneault; F Shareck; R Morosoli; D Kluepfel
Journal:  Biochem J       Date:  1996-11-01       Impact factor: 3.857

Review 5.  Esterases of xylan-degrading microorganisms: production, properties, and significance.

Authors:  L P Christov; B A Prior
Journal:  Enzyme Microb Technol       Date:  1993-06       Impact factor: 3.493

6.  Purification and characterization of ferulate and p-coumarate decarboxylase from Bacillus pumilus.

Authors:  G Degrassi; P Polverino De Laureto; C V Bruschi
Journal:  Appl Environ Microbiol       Date:  1995-01       Impact factor: 4.792

7.  Purification and properties of an acetylxylan esterase from Fibrobacter succinogenes S85.

Authors:  K P McDermid; C W Forsberg; C R MacKenzie
Journal:  Appl Environ Microbiol       Date:  1990-12       Impact factor: 4.792

8.  Isolation of two beta-xylosidase genes of Bacillus pumilus and comparison of their gene products.

Authors:  W Panbangred; O Kawaguchi; T Tomita; A Shinmyo; H Okada
Journal:  Eur J Biochem       Date:  1984-01-16

9.  Molecular cloning of the genes for xylan degradation of Bacillus pumilus and their expression in Escherichia coli.

Authors:  W Panbangred; T Kondo; S Negoro; A Shinmyo; H Okada
Journal:  Mol Gen Genet       Date:  1983

10.  Purification and some characteristics of the acetylxylan esterase from Schizophyllum commune.

Authors:  N Halgasová; E Kutejová; J Timko
Journal:  Biochem J       Date:  1994-03-15       Impact factor: 3.857
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  7 in total

1.  Structural Characterization and Directed Evolution of a Novel Acetyl Xylan Esterase Reveals Thermostability Determinants of the Carbohydrate Esterase 7 Family.

Authors:  Fiyinfoluwa A Adesioye; Thulani P Makhalanyane; Surendra Vikram; Bryan T Sewell; Wolf-Dieter Schubert; Don A Cowan
Journal:  Appl Environ Microbiol       Date:  2018-04-02       Impact factor: 4.792

2.  Purification and properties of an esterase from the yeast Saccharomyces cerevisiae and identification of the encoding gene.

Authors:  G Degrassi; L Uotila; R Klima; V Venturi
Journal:  Appl Environ Microbiol       Date:  1999-08       Impact factor: 4.792

3.  Isolation and biochemical characterization of two novel metagenome-derived esterases.

Authors:  C Elend; C Schmeisser; C Leggewie; P Babiak; J D Carballeira; H L Steele; J-L Reymond; K-E Jaeger; W R Streit
Journal:  Appl Environ Microbiol       Date:  2006-05       Impact factor: 4.792

4.  Characterisation of a Novel Acetyl Xylan Esterase (BaAXE) Screened from the Gut Microbiota of the Common Black Slug (Arion ater).

Authors:  Henry Madubuike; Natalie Ferry
Journal:  Molecules       Date:  2022-05-07       Impact factor: 4.927

5.  Hyperthermostable acetyl xylan esterase.

Authors:  Katharina Drzewiecki; Angel Angelov; Meike Ballschmiter; Klaus-Jürgen Tiefenbach; Reinhard Sterner; Wolfgang Liebl
Journal:  Microb Biotechnol       Date:  2009-09-18       Impact factor: 5.813

Review 6.  Understanding the structural and functional properties of carbohydrate esterases with a special focus on hemicellulose deacetylating acetyl xylan esterases.

Authors:  Ayyappa Kumar Sista Kameshwar; Wensheng Qin
Journal:  Mycology       Date:  2018-07-04

7.  The production and characterization of a new active lipase from Acremonium alcalophilum using a plant bioreactor.

Authors:  Eridan Orlando Pereira; Adrian Tsang; Tim A McAllister; Rima Menassa
Journal:  Biotechnol Biofuels       Date:  2013-08-01       Impact factor: 6.040
  7 in total

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