| Literature DB >> 10212186 |
G Bánhegyi1, L Lusini, F Puskás, R Rossi, R Fulceri, L Braun, V Mile, P di Simplicio, J Mandl, A Benedetti.
Abstract
A bi-directional, saturable transport of glutathione (GSH) was found in rat liver microsomal vesicles. GSH transport could be inhibited by the anion transport blockers flufenamic acid and 4, 4'-diisothiocyanostilbene-2,2'-disulfonic acid. A part of GSH taken up by the vesicles was metabolized to glutathione disulfide (GSSG) in the lumen. Microsomal membrane was virtually nonpermeable toward GSSG; accordingly, GSSG generated in the microsomal lumen could hardly exit. Therefore, GSH transport, contrary to previous assumptions, is preferred in the endoplasmic reticulum, and GSSG entrapped and accumulated in the lumen creates the oxidized state of its redox buffer.Entities:
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Year: 1999 PMID： 10212186 DOI： 10.1074/jbc.274.18.12213
Source DB: PubMed Journal: J Biol Chem ISSN： 0021-9258 Impact factor: 5.157