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Literature DB >> 10211840

Disulfide engineering at the dimer interface of Lactobacillus casei thymidylate synthase: crystal structure of the T155C/E188C/C244T mutant.

S S Velanker¹, R S Gokhale, S S Ray, B Gopal, S Parthasarathy, D V Santi, P Balaram, M R Murthy.

Abstract

The crystal structure of a covalently cross-linked Lactobacillus casei thymidylate synthase has been determined at 2.8 A resolution. The sites for mutation to achieve the bis-disulfide linked dimer were identified using the disulfide modeling program MODIP. The mutant so obtained was found to be remarkably thermostable. This increase in stability has been reasoned to be entirely a consequence of the covalent gluing between the two subunits.

Entities: Chemical Mutation Species

Mesh：

Substances：
Thymidylate Synthase

Year: 1999 PMID： 10211840 PMCID： PMC2144305 DOI： 10.1110/ps.8.4.930

Source DB: PubMed Journal: Protein Sci ISSN： 0961-8368 Impact factor: 6.725

14 in total

1. Improved methods for building protein models in electron density maps and the location of errors in these models.

Authors: T A Jones; J Y Zou; S W Cowan; M Kjeldgaard
Journal: Acta Crystallogr A Date: 1991-03-01 Impact factor: 2.290

2. Cavity-creating mutation at the dimer interface of Plasmodium falciparum triosephosphate isomerase: restoration of stability by disulfide cross-linking of subunits.

Authors: B Gopal; S S Ray; R S Gokhale; H Balaram; M R Murthy; P Balaram
Journal: Biochemistry Date: 1999-01-05 Impact factor: 3.162

3. Atomic structure of thymidylate synthase: target for rational drug design.

Authors: L W Hardy; J S Finer-Moore; W R Montfort; M O Jones; D V Santi; R M Stroud
Journal: Science Date: 1987-01-23 Impact factor: 47.728

4. Engineering of an intersubunit disulphide bridge in glutathione reductase from Escherichia coli.

Authors: N S Scrutton; A Berry; R N Perham
Journal: FEBS Lett Date: 1988-12-05 Impact factor: 4.124

5. Thermal stabilization of thymidylate synthase by engineering two disulfide bridges across the dimer interface.

Authors: R S Gokhale; S Agarwalla; V S Francis; D V Santi; P Balaram
Journal: J Mol Biol Date: 1994-01-07 Impact factor: 5.469

6. Covalent reinforcement of a fragile region in the dimeric enzyme thymidylate synthase stabilizes the protein against chaotrope-induced unfolding.

Authors: R S Gokhale; S Agarwalla; D V Santi; P Balaram
Journal: Biochemistry Date: 1996-06-04 Impact factor: 3.162

7. Stereochemical modeling of disulfide bridges. Criteria for introduction into proteins by site-directed mutagenesis.

Authors: R Sowdhamini; N Srinivasan; B Shoichet; D V Santi; C Ramakrishnan; P Balaram
Journal: Protein Eng Date: 1989-11

8. Thermostabilization of the Bacillus circulans xylanase by the introduction of disulfide bonds.

Authors: W W Wakarchuk; W L Sung; R L Campbell; A Cunningham; D C Watson; M Yaguchi
Journal: Protein Eng Date: 1994-11

9. An engineered intersubunit disulfide enhances the stability and DNA binding of the N-terminal domain of lambda repressor.

Authors: R T Sauer; K Hehir; R S Stearman; M A Weiss; A Jeitler-Nilsson; E G Suchanek; C O Pabo
Journal: Biochemistry Date: 1986-10-07 Impact factor: 3.162

Disulfide engineering at the dimer interface of Lactobacillus casei thymidylate synthase: crystal structure of the T155C/E188C/C244T mutant.

1. Improved methods for building protein models in electron density maps and the location of errors in these models.

2. Cavity-creating mutation at the dimer interface of Plasmodium falciparum triosephosphate isomerase: restoration of stability by disulfide cross-linking of subunits.

3. Atomic structure of thymidylate synthase: target for rational drug design.

4. Engineering of an intersubunit disulphide bridge in glutathione reductase from Escherichia coli.

5. Thermal stabilization of thymidylate synthase by engineering two disulfide bridges across the dimer interface.

6. Covalent reinforcement of a fragile region in the dimeric enzyme thymidylate synthase stabilizes the protein against chaotrope-induced unfolding.

7. Stereochemical modeling of disulfide bridges. Criteria for introduction into proteins by site-directed mutagenesis.

8. Thermostabilization of the Bacillus circulans xylanase by the introduction of disulfide bonds.

9. An engineered intersubunit disulfide enhances the stability and DNA binding of the N-terminal domain of lambda repressor.

10. In vivo formation and stability of engineered disulfide bonds in subtilisin.

1. Trapping a 96 degrees domain rotation in two distinct conformations by engineered disulfide bridges.

2. Engineered disulfide bonds restore chaperone-like function of DJ-1 mutants linked to familial Parkinson's disease.

3. DSDBASE: a consortium of native and modelled disulphide bonds in proteins.

4. A comprehensive analysis of novel disulfide bond introduction site into the constant domain of human Fab.