Literature DB >> 10207387

Biallelic polymorphism in the intron region of beta-tubulin gene of Cryptosporidium parasites.

I M Sulaiman1, A A Lal, M J Arrowood, L Xiao.   

Abstract

Nucleotide sequencing of polymerase chain reaction amplified intron region of the Cryptosporidium parvum beta-tubulin gene in 26 human and 15 animal isolates revealed distinct genetic polymorphism between the human and bovine genotypes. The separation of 2 genotypes of C. parvum is in agreement with our previous genotyping data based on the thrombospondin-related adhesion protein (TRAP-C2) gene, indicating these genotype characteristics are linked at 2 genetic loci. Characterization of Cryptosporidium muris and Cryptosporidium serpentis has further shown that non-parvum Cryptosporidium parasites have beta-tubulin intron sequences identical to bovine genotype of C. parvum. Thus, results of this study confirm the lineage of 2 genotypes of C. parvum at 2 genetic loci and suggest a need for extensive characterization of various Cryptosporidium spp.

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Year:  1999        PMID： 10207387

Source DB:  PubMed          Journal:  J Parasitol        ISSN： 0022-3395            Impact factor:   1.276


  9 in total

1.  Identification of species and sources of Cryptosporidium oocysts in storm waters with a small-subunit rRNA-based diagnostic and genotyping tool.

Authors:  L Xiao; K Alderisio; J Limor; M Royer; A A Lal
Journal:  Appl Environ Microbiol       Date:  2000-12       Impact factor: 4.792

2.  Cloning and sequence analysis of a highly polymorphic Cryptosporidium parvum gene encoding a 60-kilodalton glycoprotein and characterization of its 15- and 45-kilodalton zoite surface antigen products.

Authors:  W B Strong; J Gut; R G Nelson
Journal:  Infect Immun       Date:  2000-07       Impact factor: 3.441

3.  Evaluation of Cryptosporidium parvum genotyping techniques.

Authors:  I M Sulaiman; L Xiao; A A Lal
Journal:  Appl Environ Microbiol       Date:  1999-10       Impact factor: 4.792

4.  Detection of cryptosporidium and identification to the species level by nested PCR and restriction fragment length polymorphism.

Authors:  Stephane Coupe; Claudine Sarfati; Samia Hamane; Francis Derouin
Journal:  J Clin Microbiol       Date:  2005-03       Impact factor: 5.948

5.  Genetic analysis of a Cryptosporidium parvum human genotype 1 isolate passaged through different host species.

Authors:  D E Akiyoshi; X Feng; M A Buckholt; G Widmer; S Tzipori
Journal:  Infect Immun       Date:  2002-10       Impact factor: 3.441

6.  Detection and genotyping of oocysts of Cryptosporidium parvum by real-time PCR and melting curve analysis.

Authors:  Sultan Tanriverdi; Atila Tanyeli; Fikri Başlamişli; Fatih Köksal; Yurdanur Kilinç; Xiaochuan Feng; Glenda Batzer; Saul Tzipori; Giovanni Widmer
Journal:  J Clin Microbiol       Date:  2002-09       Impact factor: 5.948

Review 7.  Cryptosporidium taxonomy: recent advances and implications for public health.

Authors:  Lihua Xiao; Ronald Fayer; Una Ryan; Steve J Upton
Journal:  Clin Microbiol Rev       Date:  2004-01       Impact factor: 26.132

8.  Detection and differentiation of Cryptosporidium parasites that are pathogenic for humans by real-time PCR.

Authors:  Josef R Limor; Altaf A Lal; Lihua Xiao
Journal:  J Clin Microbiol       Date:  2002-07       Impact factor: 5.948

9.  Analysis of sequence diversity at the highly polymorphic Cpgp40/15 locus among Cryptosporidium isolates from human immunodeficiency virus-infected children in South Africa.

Authors:  Brett A Leav; Malanie R Mackay; Akudo Anyanwu; Roberta M O' Connor; Ana Maria Cevallos; Gurpreet Kindra; Nigel C Rollins; Michael L Bennish; Richard G Nelson; Honorine D Ward
Journal:  Infect Immun       Date:  2002-07       Impact factor: 3.441
  9 in total

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