OBJECTIVE AND METHOD: To compare the utility of an ELISA using 3 recombinant antigens with that of the skin biopsy to estimate incidence of infections in a sentinel cohort of individuals living in an endemic community in southern Mexico during a set of 11 subsequent ivermectin treatments. RESULTS: The apparent community prevalence of infection and microfilarial skin infection before and after 11 treatments with ivermectin plus nodulectomy were 78% and 13%, and 0.68 mf/mg and 0.04 mf/mg, respectively, as measured by skin biopsy. Of a group of 286 individuals participating in all surveys, a sentinel cohort of 42 mf and serologically negative individuals had been followed since 1994. The annual percentage of individuals becoming positive in this cohort was 24% (10/42), 28% (9/33), 0%, and 4.3% (1/23) in 1995, 1996, 1997 and 1998, respectively. Likewise, the incidence in children 5 years and under (n = 13) within this sentinel cohort was 15% (2/13), 18% (2/11), 0% and 11% (1/9), respectively. All individuals became positive to both tests simultaneously, indicating that seroconversion assessed infection incidence as accurately as skin biopsy in the sentinel group. CONCLUSION: Incidence monitoring of a sentinel cohort provides an estimation of the parasite transmission in the community; it is less costly than massive sampling, and a finger prick blood test might be more acceptable in some communities.
OBJECTIVE AND METHOD: To compare the utility of an ELISA using 3 recombinant antigens with that of the skin biopsy to estimate incidence of infections in a sentinel cohort of individuals living in an endemic community in southern Mexico during a set of 11 subsequent ivermectin treatments. RESULTS: The apparent community prevalence of infection and microfilarial skin infection before and after 11 treatments with ivermectin plus nodulectomy were 78% and 13%, and 0.68 mf/mg and 0.04 mf/mg, respectively, as measured by skin biopsy. Of a group of 286 individuals participating in all surveys, a sentinel cohort of 42 mf and serologically negative individuals had been followed since 1994. The annual percentage of individuals becoming positive in this cohort was 24% (10/42), 28% (9/33), 0%, and 4.3% (1/23) in 1995, 1996, 1997 and 1998, respectively. Likewise, the incidence in children 5 years and under (n = 13) within this sentinel cohort was 15% (2/13), 18% (2/11), 0% and 11% (1/9), respectively. All individuals became positive to both tests simultaneously, indicating that seroconversion assessed infection incidence as accurately as skin biopsy in the sentinel group. CONCLUSION: Incidence monitoring of a sentinel cohort provides an estimation of the parasite transmission in the community; it is less costly than massive sampling, and a finger prick blood test might be more acceptable in some communities.
Authors: Mario A Rodríguez-Pérez; Thomas R Unnasch; Alfredo Domínguez-Vázquez; Alba L Morales-Castro; Graciela P Peña-Flores; María E Orozco-Algarra; Juan I Arredondo-Jiménez; Frank Richards; Miguel A Vásquez-Rodríguez; Vidal García Rendón Journal: Am J Trop Med Hyg Date: 2010-07 Impact factor: 2.345
Authors: Mario A Rodríguez-Pérez; Aldo Segura Cabrera; Cristian Lizarazo Ortega; María-Gloria Basáñez; John B Davies Journal: Filaria J Date: 2007-12-18
Authors: Charles H Washington; Jeanne Radday; Thomas G Streit; Heather A Boyd; Michael J Beach; David G Addiss; Rodrigue Lovince; Maribeth C Lovegrove; Jack G Lafontant; Patrick J Lammie; Allen W Hightower Journal: Filaria J Date: 2004-05-05
Authors: Patrick J Lammie; Gary Weil; Rahmah Noordin; Perumal Kaliraj; Cathy Steel; David Goodman; Vijaya B Lakshmikanthan; Eric Ottesen Journal: Filaria J Date: 2004-09-03