| Literature DB >> 10198033 |
O Zaragoza1, C Lindley, J M Gancedo.
Abstract
External cyclic AMP (cAMP) hindered the derepression of gluconeogenic enzymes in a pde2 mutant of Saccharomyces cerevisiae, but it did not prevent invertase derepression. cAMP reduced nearly 20-fold the transcription driven by upstream activation sequence (UAS1FBP1) from FBP1, encoding fructose-1,6-bisphosphatase; it decreased 2-fold the activation of transcription by UAS2FBP1. Nuclear extracts from cells derepressed in the presence of cAMP were impaired in the formation of specific UASFBP1-protein complexes in band shift experiments. cAMP does not appear to act through the repressing protein Mig1. Control of FBP1 transcription through cAMP is redundant with other regulatory mechanisms.Entities:
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Year: 1999 PMID： 10198033 PMCID： PMC93695
Source DB: PubMed Journal: J Bacteriol ISSN： 0021-9193 Impact factor: 3.490