Literature DB >> 10196761

Human ileostomy glycoproteins as a model for small intestinal mucus to investigate adhesion of probiotics.

E M Tuomola1, A C Ouwehand, S J Salminen.   

Abstract

Human ileostomy glycoproteins were used as a model for small intestinal mucus to investigate the adhesion of 12 Lactobacillus strains, one Lactococcus strain and one Propionibacterium strain, Both probiotic and dairy strains were tested. Adhesive and non-adhesive Escherichia coli strains were used as controls. All the strains were also tested for their adhesion to polystyrene. Adhesion to ileostomy glycoproteins and to polystyrene varied significantly among the strains tested. Lactobacillus rhamnosus (human isolate), Lactobacillus GG, Lact. acidophilus 1 and P. freudenreichii adhered to ileostomy glycoproteins. Adhesion was concentration-dependent and the most adhesive strains were able to saturate the substratum. These results indicate that human ileostomy glycoproteins can be used as a model system to select potential probiotic strains to complement the adhesion test with intestinal cell lines.

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Year:  1999        PMID: 10196761     DOI: 10.1046/j.1365-2672.1999.00518.x

Source DB:  PubMed          Journal:  Lett Appl Microbiol        ISSN: 0266-8254            Impact factor:   2.858


  14 in total

1.  In vitro adhesion and platelet aggregation properties of bacteremia-associated lactobacilli.

Authors:  P V Kirjavainen; E M Tuomola; R G Crittenden; A C Ouwehand; D W Harty; L F Morris; H Rautelin; M J Playne; D C Donohue; S J Salminen
Journal:  Infect Immun       Date:  1999-05       Impact factor: 3.441

2.  GroEL of Lactobacillus johnsonii La1 (NCC 533) is cell surface associated: potential role in interactions with the host and the gastric pathogen Helicobacter pylori.

Authors:  Gabriela E Bergonzelli; Dominique Granato; Raymond D Pridmore; Laure F Marvin-Guy; Dominique Donnicola; Irène E Corthésy-Theulaz
Journal:  Infect Immun       Date:  2006-01       Impact factor: 3.441

3.  Adherence of probiotic bacteria to human intestinal mucus in healthy infants and during rotavirus infection.

Authors:  M Juntunen; P V Kirjavainen; A C Ouwehand; S J Salminen; E Isolauri
Journal:  Clin Diagn Lab Immunol       Date:  2001-03

4.  Adherence potential of indigenous lactic acid bacterial isolates obtained from fermented foods of Western Himalayas to intestinal epithelial Caco-2 and HT-29 cell lines.

Authors:  Sakshi Sharma; Sarbjit Singh Kanwar
Journal:  J Food Sci Technol       Date:  2017-08-28       Impact factor: 2.701

5.  Detection and Specific Enumeration of Multi-Strain Probiotics in the Lumen Contents and Mucus Layers of the Rat Intestine After Oral Administration.

Authors:  Hee Ji Lee; David A Orlovich; John R Tagg; J Paul Fawcett
Journal:  Probiotics Antimicrob Proteins       Date:  2009-09-16       Impact factor: 4.609

6.  Purification and characterization of a surface protein from Lactobacillus fermentum 104R that binds to porcine small intestinal mucus and gastric mucin.

Authors:  Maurilia Rojas; Felipe Ascencio; Patricia L Conway
Journal:  Appl Environ Microbiol       Date:  2002-05       Impact factor: 4.792

7.  An in vitro study on bacterial growth interactions and intestinal epithelial cell adhesion characteristics of probiotic combinations.

Authors:  Mahta Moussavi; Michelle Catherine Adams
Journal:  Curr Microbiol       Date:  2009-12-01       Impact factor: 2.188

8.  In vitro adhesion specificity of indigenous Lactobacilli within the avian intestinal tract.

Authors:  Sanna Edelman; Benita Westerlund-Wikström; Susanna Leskelä; Hannele Kettunen; Nina Rautonen; Juha Apajalahti; Timo K Korhonen
Journal:  Appl Environ Microbiol       Date:  2002-10       Impact factor: 4.792

9.  Identification of a Gene Cluster for the Biosynthesis of a Long, Galactose-Rich Exopolysaccharide in Lactobacillus rhamnosus GG and Functional Analysis of the Priming Glycosyltransferase.

Authors:  Sarah Lebeer; Tine L A Verhoeven; Grégory Francius; Geert Schoofs; Ivo Lambrichts; Yves Dufrêne; Jos Vanderleyden; Sigrid C J De Keersmaecker
Journal:  Appl Environ Microbiol       Date:  2009-04-03       Impact factor: 4.792

10.  Assessing the adhesion of putative indigenous probiotic lactobacilli to human colonic epithelial cells.

Authors:  Raj Kumar Duary; Yudhishthir Singh Rajput; Virender Kumar Batish; Sunita Grover
Journal:  Indian J Med Res       Date:  2011-11       Impact factor: 2.375

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