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Literature DB >> 1017592

Serum glycoproteins in the liver diseases. I. Studies on sialic acid-free glycoproteins-improved competitive binding assay of desialylated glycoprotein by rat liver plasma membrane.

Abstract

An improved method for quantitation of desialylated glycoproteins was developed. The assay was carried out in non-saturating condition other than saturating condition which has been used by the other authors. In the present method a lower radioactivity in the assay solution can be used. Standard curves obtained by adding an increasing amount of desialylated alpha1-acid glycoprotein to the assay solution made it possible to measure desialylated glycoproteins equivalent to 5-200 ng of desialylated alpha1-acid glycoprotein. Desialylated alpha1-acid glycoprotein used for the assay could be prepared in large scale by hydrolysis with 0.1 N-H2SO4.

Entities: Chemical Disease Species

Mesh：

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Year: 1976 PMID： 1017592

Source DB: PubMed Journal: Gastroenterol Jpn ISSN： 0435-1339

14 in total

1. Further studies on an eleventh case of heavy (Hgamma1) chain disease--physico-chemical studies.

Authors: T Arima; C Miyamoto-Sudo; M Hiroata; T Tanigawa; S Tsuboi
Journal: Acta Med Okayama Date: 1975-06 Impact factor: 0.892

2. A SENSITIVE DOUBLE ANTIBODY IMMUNOASSAY FOR HUMAN GROWTH HORMONE IN PLASMA.

Authors: D S SCHALCH; M L PARKER
Journal: Nature Date: 1964-09-12 Impact factor: 49.962

3. Physical and chemical studies on ceruloplasmin. IX. The role of galactosyl residues in the clearance of ceruloplasmin from the circulation.

Authors: C J Van Den Hamer; A G Morell; I H Scheinberg; J Hickman; G Ashwell
Journal: J Biol Chem Date: 1970-09-10 Impact factor: 5.157

4. Studies on the chemical and enzymatic modification of glycoproteins. A general method for the tritiation of sialic acid-containing glycoproteins.

Authors: L Van Lenten; G Ashwell
Journal: J Biol Chem Date: 1971-03-25 Impact factor: 5.157

5. A modified method for the isolation of the plasma membrane from rat liver.

Authors: T K Ray
Journal: Biochim Biophys Acta Date: 1970-01-06

6. The binding of desialylated glycoproteins by plasma membranes of rat liver. Development of a quantitative inhibition assay.

Authors: L Van Lenten; G Ashwell
Journal: J Biol Chem Date: 1972-07-25 Impact factor: 5.157

2. Ricinus communis agglutinin II-reactive glycoproteins from the ascites of patients with hepatocellular carcinoma and their use in enzyme-linked immunosorbent assay.

Authors: M Fukushima; T Arima; H Nagashima
Journal: Gastroenterol Jpn Date: 1987-04

6. Serum glycoproteins in the liver diseases. VI. The presence of desialylated glycoprotein binding activity in particulate fraction of human liver homogenate.

Authors: T Arima; T Kondo; H Nagashima
Journal: Gastroenterol Jpn Date: 1978

6 in total

Serum glycoproteins in the liver diseases. I. Studies on sialic acid-free glycoproteins-improved competitive binding assay of desialylated glycoprotein by rat liver plasma membrane.

1. Further studies on an eleventh case of heavy (Hgamma1) chain disease--physico-chemical studies.

2. A SENSITIVE DOUBLE ANTIBODY IMMUNOASSAY FOR HUMAN GROWTH HORMONE IN PLASMA.

3. Physical and chemical studies on ceruloplasmin. IX. The role of galactosyl residues in the clearance of ceruloplasmin from the circulation.

4. Studies on the chemical and enzymatic modification of glycoproteins. A general method for the tritiation of sialic acid-containing glycoproteins.

5. A modified method for the isolation of the plasma membrane from rat liver.

6. The binding of desialylated glycoproteins by plasma membranes of rat liver. Development of a quantitative inhibition assay.

7. Serum placental lactogen levels during pregnancy and in trophoblastic disease.

8. Catabolism of desialylated ceruloplasmin in the liver.

9. The role of sialic acid in determining the survival of glycoproteins in the circulation.

10. Radioimmunoassay of human thyroxine-binding globulin (TBG).

1. Serum glycoproteins in the liver diseases. III. Desialylated glycoproteins in the acute hepatitis.

2. Ricinus communis agglutinin II-reactive glycoproteins from the ascites of patients with hepatocellular carcinoma and their use in enzyme-linked immunosorbent assay.

3. [Glycoproteins: their biological and clinical significance. II (author's transl)].

4. Serum glycoproteins in the liver diseases. VIII. Desialylated glycoproteins in the liver cirrhosis.

5. Serum glycoproteins in the liver diseases. V. Desialylated glycoproteins in chronic hepatitis.

6. Serum glycoproteins in the liver diseases. VI. The presence of desialylated glycoprotein binding activity in particulate fraction of human liver homogenate.