Literature DB >> 1016168

A standardised haemagglutination inhibition test for porcine parvovirus antibody.

H S Joo, C R Donaldson-Wood, R H Johnson.   

Abstract

Basic variables of the haemagglutination inhibition (HI) test for porcine parvovirus antibody were investigated. Nonspecific serum inhibitors were satisfactorily removed without loss of specific antibody when undiluted serum was adsorbed with 25 percent kaolin in borate saline at pH 9.0. Natural haemagglutinins in test serums could be completely removed using 0.1 ml of packed erythrocytes to 0.6 ml of kaolin treated serums. Adsorption of prediluted serum resulted in a depression of specific antibody titres. Highest HI titres were obtained using guinea pig erythrocytes, following incubation of virus-serum mixtures for 18 hours at 4 degrees C, 3 hours at 25 degrees C or 2 hours at 37 degrees C. Micro- and macro-tests gave comparable HI titres.

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Year:  1976        PMID： 1016168     DOI： 10.1111/j.1751-0813.1976.tb09517.x

Source DB:  PubMed          Journal:  Aust Vet J        ISSN： 0005-0423            Impact factor:   1.281


  21 in total

1.  Parvovirus diagnostics and vaccine production in insect cells.

Authors:  J I Casal
Journal:  Cytotechnology       Date:  1996-01       Impact factor: 2.058

2.  Cloned genomic DNA of type 2 porcine circovirus is infectious when injected directly into the liver and lymph nodes of pigs: characterization of clinical disease, virus distribution, and pathologic lesions.

Authors:  M Fenaux; P G Halbur; G Haqshenas; R Royer; P Thomas; P Nawagitgul; M Gill; T E Toth; X J Meng
Journal:  J Virol       Date:  2002-01       Impact factor: 5.103

3.  Cryopreserved erythrocytes in clinical laboratory hemagglutination and hemagglutination inhibition tests.

Authors:  R K Maes; M M Hayes; J P Newman
Journal:  J Clin Microbiol       Date:  1985-10       Impact factor: 5.948

4.  Porcine parvovirus: replication in and inhibition of selected cellular functions of swine alveolar macrophages and peripheral blood lymphocytes.

Authors:  M J Harding; T W Molitor
Journal:  Arch Virol       Date:  1988       Impact factor: 2.574

5.  [The influence of previous infections on the development of experimental swine pleuropneumonia in specific pathogen free pigs].

Authors:  P Marois; E Di Franco; G Boulay; P Flipot; R Assaf; G Lamoureux
Journal:  Can J Vet Res       Date:  1989-10       Impact factor: 1.310

6.  Immunogenicity and pathogenicity of chimeric infectious DNA clones of pathogenic porcine circovirus type 2 (PCV2) and nonpathogenic PCV1 in weanling pigs.

Authors:  M Fenaux; T Opriessnig; P G Halbur; X J Meng
Journal:  J Virol       Date:  2003-10       Impact factor: 5.103

7.  Porcine parvovirus: virus purification and structural and antigenic properties of virion polypeptides.

Authors:  T W Molitor; H S Joo; M S Collett
Journal:  J Virol       Date:  1983-02       Impact factor: 5.103

8.  Identification and characterization of a porcine parvovirus nonstructural polypeptide.

Authors:  T W Molitor; H S Joo; M S Collett
Journal:  J Virol       Date:  1985-09       Impact factor: 5.103

9.  KBSH parvovirus: comparison with porcine parvovirus.

Authors:  T W Molitor; H S Joo; M S Collett
Journal:  J Virol       Date:  1985-08       Impact factor: 5.103

10.  Enzootic pneumonia in feeder pigs: Observations on causal factors.

Authors:  E Difranco; P Marois; J P Descôteaux; M Lacroix; P Flipot
Journal:  Can Vet J       Date:  1989-03       Impact factor: 1.008
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