An in-vitro study of H 2O 2-treated titanium surfaces in contact with blood plasma and a simulated body fluid.

Ellipsometry and antibody techniques were used to investigate plasma protein adsorption onto titanium (Ti) surfaces pretreated in hydrogen peroxide (H 2O 2). Surfaces preincubated for short times in 10 mM or 100 mM H 2O 2 bound relatively large amounts of anti-high molecular weight kininogen (a-HMWK) after immersion in blood plasma. Increasing the preincubation time in H 2O 2 led to an increase in the total amount of bound plasma proteins and a large deposition of anti-fibrinogen (a-Fib). Large amounts of a-HMWK and a-Fib were also deposited onto surfaces washed in trichloroethane, acetone and ethanol, whereas radiofrequency plasma-treated surfaces or surfaces incubated in deionized water bound preferentially a-HMWK after plasma immersion. Auger electron spectroscopy (AES) made on Ti-surfaces preincubated in 10 mM and 100 mM H 2O 2 solutions showed an increased oxide thickness and, after 16 h of immersion in a physiological buffer, an increased amount of calcium on and throughout the oxide. The rate of net oxide growth was larger in 10 mM than in 100 mM H 2O 2.