Literature DB >> 101209

Immunochemical characterization of casein from rabbit mammary gland.

K Al-Sarraj, D A White, R J Mayer.   

Abstract

1. Excellent precipitating antibodies to rabbit recombined casein polypeptides were obtained in a sheep after 8 weeks of immunization with rabbit recombined polypeptides coupled to Sepharose-albumin. 2. The antiserum was assessed for specificity by several immunochemical techniques and was monospecific when tested against acid-precipitated casein, recombined casein and extracts of lactating rabbit mammary tissue. 3. A specific anti-casein immunoglobulin fraction was prepared by immunoadsorption of the antiserum by using Sepharose-recombined casein as immunoadsorbent. 4. The specific anti-casein immunoglobulin was used to prepare a Sepharose-anti-casein immunoadsorbent for the isolation of casein from extracts of rabbit mammary tissue.

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Year:  1978        PMID: 101209      PMCID: PMC1185854          DOI: 10.1042/bj1730877

Source DB:  PubMed          Journal:  Biochem J        ISSN: 0264-6021            Impact factor:   3.857


  13 in total

1.  An indirect radioimmunoassay for mouse casein using 125I-labeled antigen.

Authors:  P M Terry; E M Ball; R Ganguly; M R Banerjee
Journal:  J Immunol Methods       Date:  1975-12       Impact factor: 2.303

2.  Absorption of antisera for studies on specific enzyme turnover.

Authors:  J H Walker; S A Betts; R Manning; R J Mayer
Journal:  Biochem J       Date:  1976-11       Impact factor: 3.857

3.  Purification of the mRNAs for Ewe alphaS-casein and beta-casein by immunoprecipitation of polysomes.

Authors:  L M Houdebine; P Gaye
Journal:  Eur J Biochem       Date:  1976-03-16

4.  Regulation of casein synthesis in the rabbit mammary gland. Titration of mRNA activity for casein under prolactin and progesterone treatments.

Authors:  L M Houdebine; P Gaye
Journal:  Mol Cell Endocrinol       Date:  1975-07       Impact factor: 4.102

5.  Regulation of casein messenger RNA during the development of the rat mammary gland.

Authors:  J M Rosen; S L Woo; J P Comstock
Journal:  Biochemistry       Date:  1975-07       Impact factor: 3.162

6.  Protein measurement with the Folin phenol reagent.

Authors:  O H LOWRY; N J ROSEBROUGH; A L FARR; R J RANDALL
Journal:  J Biol Chem       Date:  1951-11       Impact factor: 5.157

7.  Phospholipase A 1 activity of guinea pig pancreas.

Authors:  D A White; D J Pounder; J N Hawthorne
Journal:  Biochim Biophys Acta       Date:  1971-07-21

8.  A direct radioimmunoassay for mouse casein.

Authors:  M K Feldman
Journal:  Comp Biochem Physiol A Comp Physiol       Date:  1974-09-01

9.  Guinea-pig milk-protein synthesis. Isolation and characterization of messenger ribonucleic acids from lactating mammary gland and identification of caseins and pre-alpha-lactalbumin as translation products in heterologous cell-free systems.

Authors:  R K Craig; P A Brown; O S Harrison; D McIlreavy; P N Campbell
Journal:  Biochem J       Date:  1976-10-15       Impact factor: 3.857

10.  Regulation of enzyme turnover during tissue differention. Studies on the effects of hormones on the turnover of fatty acid synthetase in rabbit mammary gland in organ culture.

Authors:  B K Speake; R Dils; R J Mayer
Journal:  Biochem J       Date:  1975-05       Impact factor: 3.857

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  7 in total

1.  Immunochemical characterization of monoamine oxidase from human liver, placenta, platelets and brain cortex.

Authors:  S M Russell; J Davey; R J Mayer
Journal:  Biochem J       Date:  1979-07-01       Impact factor: 3.857

2.  Chemical and immunochemical characterization of caseins and the major whey proteins of rabbit milk.

Authors:  R Dayal; J Hurlimann; Y M Suard; J P Kraehenbuhl
Journal:  Biochem J       Date:  1982-01-01       Impact factor: 3.857

3.  Protein degradation during terminal cytodifferentiation. Studies on mammary gland in organ culture.

Authors:  C J Wilde; N Paskin; J Saxton; R J Mayer
Journal:  Biochem J       Date:  1980-10-15       Impact factor: 3.857

4.  The effect of tunicamycin on the glycosylation of lactating-rabbit mammary glycoproteins.

Authors:  B K Speake; D A White
Journal:  Biochem J       Date:  1979-06-15       Impact factor: 3.857

5.  Extensive destruction of newly synthesized casein in mammary explants in organ culture.

Authors:  H Razooki Hasan; D A White; R J Mayer
Journal:  Biochem J       Date:  1982-01-15       Impact factor: 3.857

6.  Glycoprotein synthesis in explants of developing rabbit mammary gland in culture.

Authors:  J P Bradshaw; D A White
Journal:  Biochem J       Date:  1981-09-15       Impact factor: 3.857

7.  Casein turnover in rabbit mammary explants in organ culture.

Authors:  K Al-Sarraj; J Newbury; D A White; R J Mayer
Journal:  Biochem J       Date:  1979-09-15       Impact factor: 3.857

  7 in total

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