| Literature DB >> 1010938 |
L A Aarden, E R De Groot, F Lakmaker.
Abstract
DNA/anti-DNA complexes were studied by equilibrium density centrifugation in CsC1 and by zonal centrifugation in sucrose gradients. Complexes prepared of circular DNA preparations PM2 DNA or SV40 DNA with anti-DNA obtained from patients with systemic lupus erythematosus were found to be stable under the conditions of CsC1 equilibrium centrifugation. This enabled homogeneous complexes of DNA and anti-DNA of composition AgAb1, AgAb2, AgAb3 etc to be isolated. The stability of these complexes, their banding pattern in CsC1 gradients and their sedimentation behaviour suggest that anti-DNA binds to DNA in a monogamous bivalent way.Entities:
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Year: 1976 PMID： 1010938 DOI： 10.1016/0022-1759(76)90071-5
Source DB: PubMed Journal: J Immunol Methods ISSN： 0022-1759 Impact factor: 2.303