Literature DB >> 10101301

Characterization, cDNA cloning, and functional expression of mouse ileal sodium-dependent bile acid transporter.

T Saeki1, K Matoba, H Furukawa, K Kirifuji, R Kanamoto, K Iwami.   

Abstract

Mouse ileal sodium dependent bile acid transporter (ISBT) was characterized using isolated enterocytes. Only enterocytes from the most distal portion showed Na+-dependent [3H]taurocholate uptake. Northern blot analysis using a probe against mouse ISBT revealed the expression of mouse ISBT mRNA to be restricted to the distal ileum. The Km and Vmax for Na+-dependent [3H]taurocholate transport into isolated ileocytes were calculated as 27 microM and 360 pmol/mg protein/min, respectively. Uptake of [3H]taurocholate was inhibited by N-ethylmaleimide. We have cloned ISBT cDNA from mouse ileum. The cDNA included the entire open reading frame coding 348 amino acid protein with seven hydrophobic segments and two N-glycosylation sites. COS-7 cells transfected with the expression vector containing this cDNA expressed Na+-dependent [3H]taurocholate uptake activity with a Km of 34 microM.

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Year:  1999        PMID： 10101301     DOI： 10.1093/oxfordjournals.jbchem.a022358

Source DB:  PubMed          Journal:  J Biochem        ISSN： 0021-924X            Impact factor:   3.387


  13 in total

Review 1.  Intestinal Absorption of Bile Acids in Health and Disease.

Authors:  Alexander L Ticho; Pooja Malhotra; Pradeep K Dudeja; Ravinder K Gill; Waddah A Alrefai
Journal:  Compr Physiol       Date:  2019-12-18       Impact factor: 9.090

Review 2.  Regulation of the ileal bile acid-binding protein gene: an approach to determine its physiological function(s).

Authors:  Jean-François Landrier; Jacques Grober; Isabelle Zaghini; Philippe Besnard
Journal:  Mol Cell Biochem       Date:  2002-10       Impact factor: 3.396

Review 3.  Bile acid transporters: structure, function, regulation and pathophysiological implications.

Authors:  Waddah A Alrefai; Ravinder K Gill
Journal:  Pharm Res       Date:  2007-04-03       Impact factor: 4.200

Review 4.  Apical sodium dependent bile acid transporter (ASBT, SLC10A2): a potential prodrug target.

Authors:  Anand Balakrishnan; James E Polli
Journal:  Mol Pharm       Date:  2006 May-Jun       Impact factor: 4.939

5.  Transport of bile acids in multidrug-resistance-protein 3-overexpressing cells co-transfected with the ileal Na+-dependent bile-acid transporter.

Authors:  Noam Zelcer; Tohru Saeki; Ilse Bot; Annemieke Kuil; Piet Borst
Journal:  Biochem J       Date:  2003-01-01       Impact factor: 3.857

6.  The organic solute transporter alpha-beta, Ostalpha-Ostbeta, is essential for intestinal bile acid transport and homeostasis.

Authors:  Anuradha Rao; Jamie Haywood; Ann L Craddock; Martin G Belinsky; Gary D Kruh; Paul A Dawson
Journal:  Proc Natl Acad Sci U S A       Date:  2008-02-21       Impact factor: 11.205

Review 7.  Bile acid transporters.

Authors:  Paul A Dawson; Tian Lan; Anuradha Rao
Journal:  J Lipid Res       Date:  2009-06-04       Impact factor: 5.922

Review 8.  The sodium bile salt cotransport family SLC10.

Authors:  Bruno Hagenbuch; Paul Dawson
Journal:  Pflugers Arch       Date:  2003-07-08       Impact factor: 3.657

Review 9.  The solute carrier family SLC10: more than a family of bile acid transporters regarding function and phylogenetic relationships.

Authors:  J Geyer; T Wilke; E Petzinger
Journal:  Naunyn Schmiedebergs Arch Pharmacol       Date:  2006-03-16       Impact factor: 3.000

10.  Unique inhibition of bile salt-induced apoptosis by lecithins and cytoprotective bile salts in immortalized mouse cholangiocytes.

Authors:  Daisuke Komichi; Susumu Tazuma; Tomoji Nishioka; Hideyuki Hyogo; Mizuho Une; Kazuaki Chayama
Journal:  Dig Dis Sci       Date:  2003-12       Impact factor: 3.199
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