Literature DB >> 1009928

Endoribonuclease IV. A poly(A)-specific ribonuclease from chick oviduct. 1. Purification of the enzyme.

W E Müller.   

Abstract

A new endoribonuclease, termed endoribonuclease IV, has been described. This enzyme has been isolated from chick oviducts and purified 15 000-fold in a 25% yield nearly to homogeneity. The nuclease, which specifically degrades poly(A), forms oligonucleotides of an average chain length of 10. These (A)-10 fragments are terminated by 3'-hydroxyl and 5'-phosphate groups. The enzyme has a pH optimum at 8.7, requires Mn2+ or Mg2+ as a cofactor, and has a molecular weight of about 45 000.

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Year:  1976        PMID： 1009928     DOI： 10.1111/j.1432-1033.1976.tb10975.x

Source DB:  PubMed          Journal:  Eur J Biochem        ISSN： 0014-2956


  8 in total

1.  Immunosuppressive function of hepatitis B antigens in vitro: role of endoribonuclease V as one potential trans inactivator for cytokines in macrophages and human hepatoma cells.

Authors:  C Jochum; R Voth; S Rossol; K H Meyer zum Büschenfelde; G Hess; H Will; H C Schröder; R Steffen; W E Müller
Journal:  J Virol       Date:  1990-05       Impact factor: 5.103

2.  Increase in the levels of activity of polyadenylic acid-metabolizing enzymes following phytohaemagglutinin stimulation of human lymphocytes.

Authors:  N C Courtis; T T Trangas; C M Tsiapalis
Journal:  Mol Cell Biochem       Date:  1987-05       Impact factor: 3.396

3.  Permeability measurements with closed vesicles from rat liver nuclear envelopes.

Authors:  N Riedel; M Bachmann; D Prochnow; H P Richter; H Fasold
Journal:  Proc Natl Acad Sci U S A       Date:  1987-06       Impact factor: 11.205

4.  Influence of the xyloadenosine analogue of 2',5'-oligoriboadenylate on poly(A)-specific, 2',5'-oligoriboadenylate degrading 2',3'-exoribonuclease and further enzymes involved in poly(A)(+)mRNA metabolism.

Authors:  H C Schröder; G Gosselin; J L Imbach; W E Müller
Journal:  Mol Biol Rep       Date:  1984-12       Impact factor: 2.316

5.  Nonadenylylated mRNA is present as polyadenylylated RNA in nuclei of Drosophila.

Authors:  J L Zimmerman; D L Fouts; L S Levy; J E Manning
Journal:  Proc Natl Acad Sci U S A       Date:  1982-05       Impact factor: 11.205

Review 6.  Modulation of poly(A)(+)mRNA-metabolizing and transporting systems under special consideration of microtubule protein and actin.

Authors:  W E Müller; A Bernd; H C Schröder
Journal:  Mol Cell Biochem       Date:  1983       Impact factor: 3.396

7.  Interaction of polyribosomal components and polyribonucleotides with microtubule proteins.

Authors:  H C Schröder; A Bernd; R K Zahn; W E Müller
Journal:  Mol Biol Rep       Date:  1982-11-30       Impact factor: 2.316

8.  Filter paper disk techniques for assay of nucleotidase.

Authors:  W E Müller; H C Schröder; J Arendes; R K Zahn; K Dose
Journal:  Mol Biol Rep       Date:  1977-09       Impact factor: 2.316
  8 in total

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